摘要
旨在建立在体测定骨骼肌收缩特性的方法,用于评价琥珀酸对C57BL/6J小鼠胫骨前肌收缩性能的影响。试验以C57BL/6J小鼠为研究模型,制作离体胫骨前肌、在体坐骨神经-腓肠肌和坐骨神经-胫骨前肌样本,对比不同方法、不同周龄和不同肌肉最大张力和张力半衰时间差异。随后,选取12只6周龄雌性小鼠,按体质量随机分为2组(n=6),分别在饮水中添加0和1%琥珀酸盐,单笼饲养,自由采食及饮水,12 h循环光照,每周记录采食量及体质量。饲养结束后,采用所建立的在体法检测胫骨前肌最大张力、张力半衰时间,以及肌肉中葡萄糖、乳酸含量和乳酸脱氢酶、琥珀酸脱氢酶活性。在体法检测胫骨前肌收缩持久性显著高于离体法。用在体法评价不同日龄、不同部位肌肉发现,腓肠肌的最大张力和张力半衰减时间均极显著高于胫骨前肌(P<0.01),8周小鼠胫骨前肌的最大张力半衰时间极显著短于20周小鼠(P<0.01),说明已成功建立在体测定肌肉收缩特性的方法。随后的饲养试验表明,虽然饮水添加1%琥珀酸盐对小鼠生长性能无显著影响,但采用在体测定肌肉收缩特性发现,琥珀酸盐显著提高了小鼠胫骨前肌的张力半衰时间和收缩后肌肉中琥珀酸脱氢酶的活性(P<0.05),显著降低了收缩后肌肉中的乳酸含量(P<0.05)。实验成功建立了在体检测骨骼肌收缩特性的评价方法,并发现饮水添加琥珀酸能增强骨骼肌抗疲劳性。研究结果对于完善骨骼肌类型转化评价方法,研制改善骨骼肌运动机能和畜禽胴体品质的新型营养素提供依据。
The main purpose of this paper is to establish an in vivo method to determine the contraction properties of skeletal muscle and evaluate the effect of succinate on the contraction of tibialis anterior muscle in C57BL/6J mice.In this experiment,in vitro and in vivo models of isolated tibial anterior or gastrocnemius muscles of C57BL/6J mice of different ages were used to test the maximum muscle tension and half-life time.Then,twelve 6-week-old female mice were randomly divided into two groups according to their body weights(n=6).0 and 1% of succinate were added into the drinking water respectively.The mice were housed in single cages with 12 h dark/light cycle,and free access to feed and drinking water.Their feed intake and body weights were recorded every 2 weeks.At the end of the experiment,the maximum tension,half-life time of tension,the content of glucose and lactic acid,the activity of lactate dehydrogenase and succinate dehydrogenase in the tibialis anterior muscle were measured in vivo. The contraction durability of the tibialis anterior muscle measured by in vivo method was significantly higher than that measured by ex-vivo method. The contraction properties of different muacles were compared with in vivo method and it was found that the maximum tension and half-attenuation time of the gastrocnemius muscle were significantly higher than that of the anterior tibial muscle(P<0.01).In addition,the maximum tension half-life time of the anterior tibial muscle was significantly shorter in 8-week mice than that in 20-week mice(P<0.01).This data demonstrated that in vivo method was successful to evaluate muscle contraction characteristics.The subsequent experiment showed that although the addition of 1% succinate to drinking water had no significant effect on the growth performance of mice,succinate significantly increased the tension half-life of the tibialis anterior muscle and the succinate dehydrogenase activity,while significantly reduced the lactic acid content in the muscle after contraction(P<0.05).This experiment successfully established an in vivo method to evaluate skeletal muscle contraction.Based on this model,it was found that succinate significantly enhanced the fatigue resistance of skeletal muscle.This paper sheds further light on the novel methods to evaluate skeletal muscle type transition.These data are also of great value for developing new nutrients to increase skeletal muscle function and carcass quality.
作者
李忠鑫
汪滔
彭文桐
王志康
尹聪
解培培
束刚
高萍
LI Zhong-xin;WANG Tao;PENG Wen-tong;WANG Zhi-kang;YIN Cong;XIE Pei-pei;SHU Gang;GAO Ping(Guangdong Provincial Key Laboratory of Animal Nutrition Control,College of Animal Sciences,South China Agricultural University,Guangzhou,Guangdong,P.R.China 510642,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2019年第6期1152-1158,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家重点研发计划项目（2018YFD0500403-3）
广东省重点领域研发计划项目（2019B020218001）
广东省普通高校创新团队项目（2017KCXTD002）~~
关键词
在体骨骼肌
琥珀酸
肌纤维类型
收缩特性
in vivo skeletal muscle
succinate
muscle fiber type
contractile characteristics
