摘要
采用ABTS和DPPH方法评价野菊Chrysanthemum indicum水提物和醇提物的抗氧化活性;选用HepG2细胞,通过乙醇诱导损伤,检测野菊水提物和醇提物处理后细胞存活率评价其保肝效果。结果表明,野菊醇提物抗氧化能力优于水提物,其中,野菊醇提物对ABTS自由基清除作用较好。酒精性肝损伤细胞活性测定显示,5、10、20μg·mL^-1的野菊水提物和醇提物处理组较乙醇损伤组(eth)细胞的存活率显著上升,总体呈现剂量依赖性,其中野菊醇提物保肝效果更优。
ABTS and DPPH methods were used to evaluate antioxidant activity of Chrysanthemum indicum aqueous extract and alcohol extract.Ethanol was used to induce damage of HepG2 cells.The survival rate of HepG2 cells after treatment with C.indicum extract was used to evaluate the liver protective effect.The results showed that the antioxidant ability of ethanol extract was better than that of water extract.Ethanol extract of C.indicum had a good scavenging effect on ABTS.The cell viability assay of alcoholic liver injury showed that the survival rates of the 5,10,20μg·mL^-1 treatment groups of C.indicum aqueous extract and alcohol extract were all significantly higher than that of the ethanol injury group(eth).The survival rate exhibited dose dependence.The ethanol extract of C.indicum had a more significant liver protective effect.
作者
梁诗
李煊
陈良华
黄雯
许传俊
童庆宣
林毅
明艳林
LIANG Shi;LI Xuan;CHEN Liang-hua;HUANG Wen;XU Chuan-jun;TONG Qing-xuan;LIN Yi;MING Yan-lin(Xiamen Overseas Chinese Subtropical Plant Introduction Garden/Plant Introduction&Quarantine and Plant Product Key Laboratory of Xiamen City,Xiamen 361002,Fujian China;Institute of Chemical Engineering,Huaqiao University,Xiamen 361021,Fujian China;Fujian Institute of Subtropical Botany/Fujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry,Xiamen 361006,Fujian China)
出处
《亚热带植物科学》
2019年第4期329-333,共5页
Subtropical Plant Science
基金
厦门市科技计划项目(3502Z20162011)
关键词
野菊
提取物
抗氧化
酒精性肝损伤
Chrysanthemum indicum
extract
antioxidant
alcoholic liver injury
