摘要
目的调查石渠县啮齿类动物构成及其棘球蚴的感染状况,为当地的棘球蚴病防治提供科学依据。方法于2015年7-8月在四川省石渠县云波沟设置4个间距为500 m的样方(0.25ha2)捕捉啮齿类动物,通过形态学方法进行物种鉴定,采用分子生物学技术检测啮齿类动物棘球蚴感染情况。结果共捕获啮齿类动物368只,其中高原鼠兔占19.0%(70/368),长尾仓鼠占0.5%(2/368),青海田鼠占74.2%(273/368),白尾松田鼠占5.4%(20/368),肢体残缺无法分类鉴定的田鼠占0.8%(3/368)。啮齿类动物DNA检测棘球蚴感染率为4.9%(95%CI:2.7%~7.1%),其中多房棘球蚴感染率为0.3%(0-0.8%),石渠棘球绦虫感染率为4.6%(2.5%~6.8%),未检测到细粒棘球蚴感染的个体。t检验显示4个样方中青海田鼠的捕获数均显著多于高原鼠兔(t=-3.431,P<0.05)。χ^2检验显示高原鼠兔和青海田鼠石渠棘球蚴感染率显著高于多房棘球蚴感染率(χ^2=值分别为14.578和7.515,均P<0.05),兔形目动物棘球蚴感染率显著高于啮齿目动物棘球蚴感染率(χ^2=4.850,P<0.05)。结论在石渠县青海田鼠和高原鼠兔是主要啮齿类物种和棘球蚴的主要野外中间宿主。在包虫病的防治中应加强对这两种物种的检测,控制棘球蚴病的传播。
Objective To conduct an investigation of the species of small mammals and the prevalence of Echinococcus spp.in order to provide a scientific basis for the prevention and control of hydatid diseases in Shiqu County,Sichuan Province.Methods From July to August 2015,four sampling quadrants(0.25 ha2)at 500-m intervals were established to capture small mammals in Yunbo Gou,Shiqu County,Sichuan Province.Host species of small mammals were identified morphologically,and the prevalence of Echinococcus spp.was detected using molecular PCR techniques.Results A total of 368 small mammals were captured,19.0%(70/368)of which were Ochotona curzoniae,0.5%(2/368)of which were Cricetulus longicaudatus,74.2%of which were Lasiopodomys fuscus(273/368),and 5.4%(20/368)of which were Phaiomys leucurus.Three vole samples(0.8%,3/368)were lost,so their species could not be identified.The overall prevalence of Echinococcus spp.detected via DNA of the cox1 and nad1 genes in small mammals was 4.9%(95%CI:2.7%-7.1%).The prevalence of E.multilocularis was 0.3%(0.0-0.8%),and the prevalence of E.shiquicus was 4.6%(2.5%-6.8%).No individuals infected with E.granulosus were detected.The t test indicated that the number of L.fuscus(78,106,41,48)captured was significantly higher than the O.curzoniae(7,25,19,19)in four quadrats(t=-40.052,P<0.05).The prevalence of E.shiquicus was 10%(3.0%-17.0%)in O.curzoniae and 3.7%(1.4-5.9%)in L.fuscus The prevalence of E.multilocularis was 0.0%in O.curzoniae and 0.4%(0-1.1%)in L.fuscus.Aχ^2 test indicated that the prevalence of E.shiquicus was significantly higher than that of E.multilocularis in both O.curzoniae and L.fuscus(χ^2=14.578,P<0.05;χ^2=7.515,P<0.05).The overall prevalence of Echinococcus spp.was 10%(3.0%-17.0%)in pikas and 3.7%(1.6%-5.8%)in rodents(χ^2=4.850,P<0.05).Conclusion The small mammal species L.fuscus and O.curzoniae were the main wild intermediate hosts of E.multilocularis and E.shiquicus in Shiqu County.More attention should be paid to L.fuscus and O.curzoniae in programs to prevent and control hydatid diseases.
作者
翁晓东
慕志强
王旭
刘佳余
孙晓慧
王正寰
WENG Xiao-dong;MU Zhi-qiang;WANG Xu;LIU Jia-yu;SUN Xiao-hui;WANG Zheng-huan(School of Life Sciences,East China Normal University,Shanghai 200062,China;National Institute of Parasitic Diseases,Center for Disease Control and Prevention)
出处
《中国病原生物学杂志》
CSCD
北大核心
2019年第11期1320-1323,共4页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.31071944,31470488)
关键词
包虫病
棘球蚴
啮齿类
石渠县
四川
Hydatid disease
echinococcus
small mammal
Shiqu County
Sichuan Province
