摘要
目的探讨DDX46基因对食管鳞癌发生发展的作用机制。方法应用基因芯片技术检测DDX46基因敲减前后食管鳞癌TE-1细胞中全基因mRNA表达丰度,筛选差异表达的基因,并对其进行生物信息学分析。对表达谱筛选的其中6个差异基因进行实时定量聚合酶链式反应(qRT-PCR)定量分析以验证结果的可靠性。结果按照表达差异倍数之绝对值≥2,且P<0.05的筛选条件,筛选出差异基因1006个,其中表达下调基因644个,表达上调基因362个。生物信息分析显示,差异基因主要涉及细胞周期、增殖、凋亡、黏附、能量代谢及免疫应答等生物学过程,磷脂酰肌醇-3激酶(PI3K)为重要节点分子。qRT-PCR检测结果与基因芯片结果的验证符合率为100%。结论利用生物信息学方法能有效挖掘DDX46敲减后食管鳞癌基因芯片数据,为进一步研究DDX46与食管鳞癌发生发展的关系,以及寻找潜在的治疗靶点提供有价值的线索。
Objective To explore the mechanism of DDX46 regulation of esophageal squamous cell carcinoma.Methods Picture signals of fluorescence in gene array were scanned and differential expression of gene in two groups(a DDX46-shRNA-LV group and a control-LV group)were compared by GCOSvL.4 software.These differential expressed genes were analyzed by bioinformatics methods finally,and validated by quantitative real time polymerase chain reaction(qRT-PCR)analysis.Results According to the screening criteria of fold change≥2 and P<0.05,1006 genes were differentially expressed after DDX46 knockdown,including 362 up-regulated and 644 down-regulated genes.Bioinformatics analysis and gene co-expression network building identified that these differentially expressed genes were mainly involved in cell cycle,proliferation,apoptosis,adhesion,energy metabolism,immune response,etc.Phosphatidylinositol 3-kinase(PI3K)was the key molecule in the network.The results of RT-qPCR were completely consistent with the results of gene microarra.Conclusion Bioinformatics can effectively exploit the microarray data of esophageal squamous cell carcinoma after DDX46 knockdown,which provides a valuable clue for further exploration of DDX46 tumorigenesis mechanism and helps to find potential drug therapy.
作者
李斌
李政
尹泚
蔺军平
杨建宝
朱多杰
冯海明
敬涛
LI Bin;LI Zheng;YIN Ci;LIN Junping;YANG Jianbao;ZHU Duojie;FENG Haiming;JING Tao(Department of Thoracic Surgery,Lanzhou University Second Hospital,Lanzhou University Second Clinical Medical College,Lanzhou,730030,P.R.China)
出处
《中国胸心血管外科临床杂志》
CAS
CSCD
2020年第1期61-67,共7页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
兰州市城关区科技计划项目（2018-9-2）
甘肃省卫生行业科研计划项目（GSWSKY2017-04）
甘肃省自然科学基金项目（1606RJZA040）
兰州大学第二医院“萃英科技创新”计划项目（CY2017-MS02）
兰州大学第二医院配套经费项目资金
关键词
DDX46
食管鳞癌
基因芯片
差异基因
生物信息学
DDX46
esophageal squamous cell carcinoma
gene chip
differential genes
bioinformatics
