摘要
目的分析微RNA(miR)检测对急性呼吸窘迫综合征(ARDS)肺损伤临床意义和相关机制。方法选取2016年4月至2019年4月间在本院术后确诊ARDS患者86例,采集患者麻醉诱导以后开始转流前(T1)、结束转流(T2)、术后8 h(T3)和术后16 h(T4)时患者动脉血,用miR微阵列芯片对ARDS患者miR改变进行分析,通过qRT-PCR验证。ELISA法检测患者T1至T4时间点时血清白细胞介素-6(IL-6)与肿瘤坏死因子-α(TNF-α)含量,T1~T44个不同时间时收集患者1 mL桡动脉行血气分析,计算氧合指数和呼吸指数;依据转染不同处理将细胞分成3组,分别是pcNDA3.1空质粒组、pcDNA3.1-miR-320组和对照组,观察3组细胞存活率和凋亡率。结果T1至T4患者血清miR-320相对表达量分别为(1.00±0.06)、(1.15±0.08)、(1.35±0.07)、(1.70±0.08),差异有统计学意义(F=20.653,P<0.05),T1至T4患者血清miR-499相对表达量分别为(1.00±0.04)、(0.95±0.06)、(0.94±0.05)、(0.95±0.04)差异无统计学意义(F=2.183,P>0.05)。T4时刻患者血清miR-320表达和呼吸指数、血清IL-6、TNF-α含量为正相关(r=0.850、0.709、0.741,P<0.05),T4时刻患者血清miR-320表达和氧合指数为负相关(r=-0.751,P<0.05)。pcDNA3.1-miR-320组细胞存活率(82.2±3.4)%低于对照组(100.0±0.0)%,细胞凋亡率(20.1±2.2)%高于对照组(9.4±1.0)%,差异有统计学意义(P>0.05);pcNDA3.1空质粒组细胞存活率(99.0±0.5)%、凋亡率(10.5±1.1)%和对照组无显著差异(P>0.05)。结论ARDS患者miR-320高表达,其作用机制可能和诱导肺泡上皮细胞凋亡有关,miR-320有评估、诊断ARDS的潜能。
Objective To analyze the clinical significance and related mechanism of microRNA detection for lung injury in ARDS.Methods Eighty-six cases of ARDS patients were selected from April 2016 to April 2019,collected arterial bloodafter induction of anesthesia started bypass(T1),bypass end(T2),after 8h(T3)and intraoperative after 16h(T4).microRNA microarray analysis of altered microRNA ARDS patients,validation by qRT-PCR.Detected by ELISA in patients with T1 to T4 time point serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)content,arterial blood gas analysis when collecting 1ml radius T1 to T4.4 different times calculated breathing indices and oxygenation index;based transfection the cells were divided into three different treatment groups,respectively pcNDA3.1 empty vector group,pcDNA3.1-miR-320 group and control group,cell viability and apoptosis were observed 3 groups rate.Results Serum miR-320 relative expression levels T1 to T4 were(1.00±0.06),(1.15±0.08),(1.35±0.07),was statistically significant(1.70±0.08)difference(F=20.653,P<0.05),T1 to T4 serum miR-499 relative expression levels were(1.00±0.04),(0.95±0.06),(0.94±0.05),(0.95±0.04)was no significant difference(F=2.183,P>0.05).Time T4 serum miR-320 expression and respiratory index,serum IL-6,TNF-αcontent is positively correlated(r=0.850,0.709,0.741,P<0.05),serum expression of miR-320 and the oxygenation index time T4 negative correlation(r=-0.751,P<0.05).pcDNA3.1-miR-320 cell viability group(82.2±3.4)%lower than the control group(100.0±0.0)%,the apoptosis rate(20.1±2.2)%control group(9.4±1.0)%,the difference statistically significant(P>0.05);pcNDA3.1 cell viability empty vector group(99.0±0.5)%,the rate of apoptosis(10.5±1.1)%was no significant difference(P>0.05)and the control group.Conclusion The mechanism of high expression of miR-320 in ARDS patients may be related to the induction of apoptosis of alveolar epithelial cells.miR-320 has the potential to evaluate and diagnose ARDS.
作者
魏建东
谭美春
姜颢
施巍
Wei Jiandong;Tan Meichun;Jiang Hao;Shi Wei(Department of Emergency Services,Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 201900,China)
出处
《山西医药杂志》
CAS
2020年第2期129-132,共4页
Shanxi Medical Journal
