摘要
目的研究辛伐他汀通过调节TGFβ1/Smad3通路对牙槽骨成骨细胞的影响。方法选择不同浓度辛伐他汀(1、10、100 nmol/L)刺激牙槽骨成骨细胞,MTT法测定24、48、72 h细胞增殖活力,流式细胞仪法测定细胞凋亡,ELISA法检测转化生长因子β1(TGFβ1)表达的变化,实时定量聚合酶链反应检测骨钙素(OC)、NF-κB配体受体激活物和骨保护素(OPG)以及细胞信号转到分子Smad3的mRNA表达水平。结果不同浓度辛伐他汀刺激成骨细胞72h后,细胞增殖活性显著升高,凋亡减少。不同浓度的辛伐他汀均能增加牙槽骨成骨细胞的OPG和转化生长因子TGF-β1表达。辛伐他汀诱导牙槽骨成骨细胞OC mRNA表达呈剂量依赖性增加。结论辛伐他汀通过调节TGF-β1/Smad3通路抑制牙槽骨成骨细胞凋亡,促进牙槽骨成骨细胞中成骨标记物的表达起到促进骨组织合成的作用。
Objective To investigate the effect of simvastatin on primary alveolar osteoblasts.Methods Different concentrations of simvastatin were used to stimulate alveolar osteoblasts.Cell proliferation was measured by MTT assay at 24,48 and 72 hours.Apoptosis was determined by flow cytometry.TGFβ1(transforming growth factorβ1)expression was examined by ELISA,and osteocalcin(OC),NF-κB ligand receptor activator(RANKL),osteoprotegerin(OPG)and mRNA expression levels of Smad3 were detected by real-time quantitative polymerase chain reaction(PCR).Results After the different concentrations of simvastatin stimulated the osteoblasts for 72h,the cell proliferation activity increased significantly,and the apoptosis decreased.Different concentrations of simvastatin increased the expression of OPG and transforming growth factor TGF-β1 in alveolar bone osteoblasts.Simvastatin induced a dose-dependent increase in osteocalcin mRNA expression in alveolar bone osteoblasts.Conclusion Simvastatin can inhibit the apoptosis of alveolar bone osteoblasts by regulating TGF-β/SMAD3 pathway,and promote bone tissue synthesis.
作者
金红
雷明辉
屠军波
JIN Hong;LEI Ming-hui;TU Jun-bo(Department of Stomatology,Second Hospital of Nuclear Industry,Xianyang 712000,China;不详)
出处
《北京口腔医学》
CAS
2019年第6期313-316,共4页
Beijing Journal of Stomatology
基金
2017-2019口腔颌面数字化诊疗技术研究与应用示范（2017ZDXM-SF-108）
