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原肌球蛋白调节蛋白1对ApoE敲除小鼠糖脂代谢的影响

Effect of Tropomodulin 1 on the glucose and lipid metabolisms of ApoE knockout mice
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摘要 目的研究原肌球蛋白调节蛋白1(Tmod1)对载脂蛋白E(ApoE)基因敲除小鼠的糖脂代谢的影响。方法将心脏过表达而全身其他部位敲除Tmod1的小鼠(TOT/Tmod1-/-)与ApoE-/-小鼠杂交获得ApoE和Tmod1的双敲小鼠(ApoE-/-/TOT/Tmod1-/-)。对ApoE-/-小鼠和双敲小鼠进行高脂饮食喂养,检测2种小鼠的体质量改变、脂肪含量、血脂水平、口服糖耐量(OGTT)和胰岛素耐量(ITT)。对肝脏和皮肤进行组织学分析。结果双敲小鼠较ApoE-/-小鼠体质量减轻,体脂含量降低(P<0.05)。但喂养高脂饮食8周后,双敲小鼠体质量增加量、皮下脂肪组织的厚度、三酰甘油和总胆固醇水平均高于ApoE-/-小鼠(P<0.05),而脂肪肝、口服葡萄糖耐量和胰岛素耐受水平则明显降低(P<0.05)。结论Tmod1参与调节高脂饮食引起的ApoE-/-小鼠糖脂代谢水平的改变。 Objective To study the effect of Tropomodulin1(Tmod1)on the glucose and lipid metabolisms of ApoE knockout mice.Methods Tmod1 knockout mice with heart specific Tmod1 overexpressing(TOT/Tmod1-/-)were bred with ApoE-/-to obtain ApoE and Tmod1 double knockout mice(ApoE-/-/TOT/Tmod1-/-).ApoE-/-and ApoE-/-/TOT/Tmod1-/-mice were fed with high-fat diet;the changes in body weight,body fat content and blood lipid were measured.The oral glucose tolerance test(OGTT)and insulin tolerance test(ITT)were performed.Histological analyses were performed on the liver and the skin.Results ApoE-/-/TOT/Tmod1-/-mice had less body weight and body fat than ApoE-/-mice(P<0.05).After fed with high-fat diet for 8 weeks,ApoE-/-/TOT/Tmod1-/-mice showed much greater body weight change,higher triglyceride and total cholesterol levels,thicker subcutaneous apdipose tissues than ApoE-/-mice(P<0.05).However,the fat deposit in the liver,the level of oral glucose tolerance as indicated by the level of glucose being relatively higher during the OGTT,and the rate of insulin-stimulated blood glucose disposal dring the ITT of ApoE-/-/TOT/Tmod1-/-mice were lower than those of ApoE-/-mice(P<0.05).Conclusion Tmod1 is involved in the regulation of glucose and lipid metabolisms of ApoE-/-mice induced by high-fat diet.
作者 文京晶 崔雨萌 雷霆雯 姚伟娟 曾柱 吴宁 WEN Jing-jing;CUI Yu-meng;LEI Ting-wen;YAO Wei-juan;ZENG Zhu;WU Ning(School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025;Hemorheology Center,Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100083,China)
机构地区 贵州医科大学基础医学院 北京大学医学部基础医学院生理与病理生理学系血液流变学研究中心
出处 《西安交通大学学报（医学版）》 CAS CSCD 北大核心 2020年第1期27-32,52,共7页 Journal of Xi’an Jiaotong University（Medical Sciences）
基金 国家自然科学基金资助项目(No.81370386,31570938)~~
关键词 载脂蛋白E(ApoE) 糖脂代谢 原肌球蛋白调节蛋白1 高脂饮食 ApoE glucose and lipid metabolisms Tropomodulin1 high-fat diet
分类号 Q493 [生物学—生理学]
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西安交通大学学报（医学版）
2020年 第1期

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