摘要
目的本文观察土荆皮乙酸(PAB)对人喉表皮样癌细胞系2(HEp-2)的增殖和凋亡的影响。方法细胞分组及处理:以0、0.5、1、2、4、8和16μmol/L的PAB分别处理细胞24、48和72 h;MTT法检测细胞活性;流式细胞仪检测细胞凋亡;Western blot和qPCR检测细胞Bcl-2、Bax和caspase-3蛋白及mRNA表达;免疫组化检测细胞PARP和c-PARP蛋白的表达。结果 PAB呈时间-浓度依赖性抑制HEp-2增殖。与对照组比较,4和8μmol/L PAB组HEp-2细胞凋亡率均显著升高(P<0.01);4和8μmol/L PAB组HEp-2细胞的Bcl-2和PARP表达均显著下降(P<0.05);Bax、caspase-3和c-PARP的表达均显著升高(P<0.05)。结论 PAB可激活线粒体凋亡途径,诱导HEp-2细胞凋亡。
Objective To investigate the effects of pseudolaric acid B(PAB) on the proliferation and apoptosis of HEp-2 cells.Methods Cell grouping and treatment: cells were treated with 0, 0.5, 1, 2, 4, 8 and 16 μmol/L PAB for 24, 48 and 72 h respectively;MTT assay was used to detect cell viability;flow cytometry was used to detect apoptosis;The expression of Bcl-2, Bax and caspase-3 protein and mRNA was detected by Western blot and qPCR. The expression of PARP and c-PARP was detected by immunohistochemistry. Results PAB inhibited HEp-2 proliferation in a time-concentration dependent manner. Compared with the control group, the apoptosis rate of HEp-2 cells in 4 μmol/L and 8 μmol/L PAB groups was significantly increased(P<0.01). Compared with the control group, the expression of Bcl-2 and PARP in HEp-2 cells of 4 μmol/L and 8 μmol/L PAB groups were significantly decreased(P<0.05), and the expression of Bax, caspase-3 and c-PARP was significantly increased(P<0.05). Conclusions PAB can activate the mitochondrial apoptosis pathway thus induce apoptosis of HEp-2 cells and inhibit cell proliferation.
作者
杨蒙生
李丽
刘雪峰
杨小龙
YANG Meng-sheng;LI Li;LIU Xue-feng;YANG Xiao-long(Department of ENT,Head and Neck Surgery,Gansu Provincial Hospital,Lanzhou 730000,China)
出处
《基础医学与临床》
CSCD
2020年第1期92-96,共5页
Basic and Clinical Medicine
关键词
土荆皮乙酸
喉癌
线粒体凋亡
机制
pseudolaric acid B
laryngeal cancer
mitochondrial apoptosis
mechanism
