摘要
为探索不同地域浓香型大曲及其曲房空气细菌群落的相关性,该文采用磷脂脂肪酸(phospholipid fatty acid,PLFA)图谱分析法对四川绵阳、泸州地区不同发酵阶段的浓香型大曲及其曲房空气细菌群落结构进行研究。结果表明,两地大曲及其曲房空气细菌群落PLFA种类数变化相反,且各阶段大曲高于对应曲房空气。两地大曲及其曲房空气细菌生物量均在后火期达到最大值,变化趋势呈先升高后降低。通过相对丰度比较和主成分分析发现,大曲优势菌群主要为嗜热解氢杆菌、革兰氏阴性菌和革兰氏阳性菌,曲房空气优势菌群主要为革兰氏阳性菌和革兰氏阴性菌,且绵阳地区大曲与曲房空气细菌群落在发酵0 d、低温期、储藏期组成相似,泸州地区大曲与曲房空气在整个发酵阶段组成相似。综上研究表明,曲房空气微生物对浓香型大曲具有重要影响且主要在发酵前期。
The purpose of this study is to inspect differences of bacterial community structure in Luzhou-flavored Daqu and in the air of the facility at different fermentation locations(Mianyang and Luzhou) using phospholipid fatty acid(PLFA) method. The results showed an opposing shifting trend in the PLFA species number in Daqu and air of the two facilities. The numbers of PLFA species in each fermentation stage was always higher than in the air. The bacterial biomass of Daqu and the air in Mianyang and Luzhou increased initially, then decreased. Both Daqu and the air reached maximum biomass at the stationary stage. Hydrogenobacter, Gram-negative and Gram-positive bacteria dominated in Daqu. However, only Gram-negative and Gram-positive bacteria predominated in the facility air. At day 0, pre-stage, and storage stage of fermentation in Mianyang, the compositions of the bacterial communities were similar in Daqu and the air. However, the compositions of bacterial community in Daqu and the air were similar during the entire fermentation process. This indicated that the microorganisms in the facility air may have influence on the Luzhou-flavor Daqu during the pre-tage of fermentation.
作者
车路萍
邓杰
卫春会
任志强
郭燕
邓波
杜礼泉
黄治国
CHE Luping;DENG Jie;WEI Chunhui;REN Zhiqiang;GUO Yan;DENG Bo;DU Liquan;HUANG Zhiguo(Liquor Making Biotechnology and Application Key Laboratory of Sichuan Province,Sichuan University of Science&Engineering,Yibin 644000,China;Luzhou Laojiao Co.,Ltd.,Luzhou 646000,China;Forgood Distillery Co.Ltd.,Mianyang 621000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第24期33-39,共7页
Food and Fermentation Industries
基金
中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2018JJ008)
固态发酵资源利用四川省重点实验室开放基金项目(2015GTY004)
酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2015-08)
关键词
不同区域
浓香型大曲
曲房空气
磷脂脂肪酸(PLFA)
细菌群落相关性
different areas
Luzhou-flavored Daqu
the air of stacking room
phospholipid fatty acid(PLFA)
bacterial community correlation
