摘要
目的:比较痛舒膏穴位贴敷与大承气汤治疗大鼠不完全性肠梗阻(IIO)的疗效,并探讨其可能机制。方法:取24只Wistar大鼠,采用贯穿缝扎末端回肠肠管的方法建立IIO大鼠模型,另选取8只健康大鼠作为正常对照组。将建模成功的24只大鼠随机分为模型组、大承气汤组和痛舒膏组,每组8只。痛舒膏组给予痛舒膏外用关元穴加艾灸治疗,大承气汤组给予大承气汤灌胃治疗,模型组和正常对照组术后均贴敷胶布。72 h后,检测血浆和小肠组织中一氧化氮(NO)水平和超氧化物歧化酶(SOD)活性,并采用苏木精—伊红(HE)染色法观察小肠组织形态学变化。结果:模型组大鼠体重较正常对照组明显下降,小肠黏膜HE染色评分增加,血浆和小肠组织NO水平升高,SOD活性降低(均P<0.05);与模型组比较,痛舒膏组和大承气汤组小肠黏膜损伤评分显著降低,血浆和小肠组织中NO水平显著降低,SOD活性显著升高(均P<0.05),而两组比较无明显差异(P>0.05)。结论:痛舒膏穴位贴敷与大承气汤对IIO大鼠的疗效相当,均能保护大鼠肠黏膜结构的完整性,减轻小肠组织损伤,其作用机制可能与降低NO含量和清除氧自由基有关。
Objective:To compare the effects of Tongshu Plaster acupoint(TPA)and Dachengqi decoction(DD)on incomplete intestinal obstruction(IIO)in rats.Methods:The IIO rats model was established by penetrating transfixion terminal ileum,and randomly divided into 3 groups:model group,TPA group and DD group,with 8 rats in each group.8 healthy rats were selected as a normal control group.The NO content and SOD activity in plasma and small intestine were detected,and the histomorphology of small intestine was observed by HE staining after 72 hours of intervention.Results:Compared with the normal control group,the body weight and the SOD activity in the model group were decreased significantly,while the HE staining score of the small intestine as well as the plasma and intestinal tissue NO level were increased(P<0.05).Compared with the model group,the intestinal mucosal injury score and the level of NO in plasma and intestinal tissues were decreased in TPA group and DD group,whereas the SOD activity was significantly increased(P<0.05),and no significant difference was found between the two groups(P>0.05).Conclusion:TPA and DD were effective in IIO rats and their efficacy was similar.They could protect the integrity of intestinal mucosal structure and reduce the damage of intestinal tissue.The mechanism might be related to the reduction of NO content and scavenging of oxygen free radicals.
作者
聂奔
陈静
龙麟
丁铌
付文胜
Nie Ben;Chen Jing;Long Lin;Ding Ni;Fu Wensheng(Oncology Department,Qingdao Haici Medical Group,Qingdao 266032,China)
出处
《广西医科大学学报》
CAS
2019年第12期1898-1902,共5页
Journal of Guangxi Medical University
基金
山东省青岛市2015年度医药科研指导计划(No.2015-WJZD039)
