摘要
目的探讨空气细颗粒物PM2.5水溶成分对人黑素细胞系PIG1增殖、迁移、酪氨酸酶活性及黑素含量的影响。方法收集采暖季雾霾天气PM2.5,制备混悬液。建立人黑素细胞系PIG1培养体系,将PIG1细胞分为实验组和对照组,其中实验组分别用10、20、50、100、200 mg/L(细胞迁移实验用10 mg/L)PM2.5混悬液处理48 h,对照组不用PM2.5处理,其他同实验组。处理完成后,采用MTT法、微孔膜法、多巴氧化法、NaOH裂解法分别检测黑素细胞的增殖、迁移、酪氨酸酶活性及黑素含量。两个样本均数间的比较采用t检验,多个样本均数间的比较采用方差分析,多个样本均数间每两个均数间的比较采用SNK法,相关性分析采用线性相关分析方法。结果与对照组[(100±1.41)%]相比,20、50、100、200 mg/L PM2.5组PIG1细胞的增殖率[分别为(93.41±2.13)%、(88.31±1.36)%、(79.75±1.89)%、(69.83±2.50)%]均受到明显抑制(均P<0.05),且随PM2.5浓度的上升,PIG1细胞的增殖率(r=-0.98,P<0.01)和酪氨酸酶活性(r=-0.93,P<0.01)均呈下降趋势。10 mg/L PM2.5处理后,黑素细胞迁移率为(66.23±1.11)%,显著低于对照组[(76.86±1.81)%,t=7.55,P<0.01]。随PM2.5浓度的升高(50~200 mg/L),PIG1细胞黑素含量逐渐减少(r=-0.97,P<0.01)。结论空气细颗粒物PM2.5可能通过抑制黑素细胞增殖、迁移和降低酪氨酸酶活性及黑素含量而影响黑素细胞的正常功能。
Objective To evaluate the effect of water-soluble components of atmospheric fine particulate matter PM2.5 on proliferation,migration,tyrosinase activity and melanin content of a human melanocyte line PIG1.Methods PM2.5 was collected during haze weather in heating seasons,and processed into suspensions.PIG1 melanocytes were cultured and divided into 5 experimental groups and 1 control group.PIG1 melanocytes in the 5 experimental groups were treated with 10,20,50,100 and 200 mg/L PM2.5 suspensions respectively for 48 hours,while cells in the control group were not treated with PM2.5 suspensions.In cell migration assay,there was only 1 experimental group treated with 10 mg/L PM2.5 suspensions.After treatment,methyl thiazol tetrazolium(MTT)assay,micropore filtration assay,DOPA oxidase assay and NaOH lysis method were performed to determine the cell proliferation rate,migration rate,tyrosinase activity and melanin content respectively.Statistical analysis was carried out by using t test for comparison of means of two samples,one-way analysis of variance for means of multiple samples,Student-Newman-Keuls(SNK)-q test for multiple comparisons,and linear correlation analysis for analysis of correlations.Results Compared with the control group([100±1.41]%),the proliferation rate of PIG1 cells significantly decreased in the 20-,50-,100-and 200-mg/L PM2.5 groups([93.41±2.13]%,[88.31±1.3557]%,[79.75±1.89]%,[69.83±2.50]%respectively,all P<0.05).Linear correlation analysis showed that the proliferation rate and tyrosinase activity of PIG1 cells decreased with the increase in PM2.5 concentrations(r=-0.98,-0.93,respectively,both P<0.01).After the treatment with 10 mg/L PM2.5,the migration rate of PIG1 cells significantly decreased(66.23%±1.11%)compared with the control group([76.86±1.81]%,t=7.55,P<0.01).With the increase in PM2.5 concentrations(50-200 mg/L),the melanin content of PIG1 cells gradually decreased(r=-0.97,P<0.01).Conclusion Atmospheric fine particulate matter PM2.5 can affect the normal functions of melanocytes by inhibiting their proliferation and migration,and reducing their tyrosinase activity and melanin content.
作者
索丹凤
曾三武
孟令贺
张峻岭
Suo Danfeng;Zeng Sanwu;Meng Linghe;Zhang Junling(Department of Dermatology,Tianjin First Central Hospital,Tianjin 300192,China;Department of Dermatology,Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China)
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2019年第12期919-922,共4页
Chinese Journal of Dermatology
基金
中华医学会-欧莱雅中国人健康皮肤研究项目(S2016-009)。
