摘要
通过不同负荷游泳训练模式,探讨细颗粒物(PM 2.5)暴露下不同运动负荷的大鼠肝脏蛋白表达功能,为PM 2.5暴露环境下有氧运动对肝脏功能的影响提供理论依据。建立PM 2.5暴露大鼠运动模型,采用随机数字表法分为PM 2.5暴露组(暴露组)、PM 2.5暴露低负荷组(LES组)、PM 2.5暴露中负荷组(MES组)、PM 2.5暴露高负荷组(HES组)。采用Elisa法检测脂肪组织肿瘤坏死因子(TNF-α)表达水平;Western blot检测过化物酶体增殖物激活受体(PPAR-γ)蛋白的表达。TNF-α检测结果显示,与其他组相比,暴露中负荷组TNF-α水平显著性降低(P<0.05);PPAR-γ检测结果显示,与暴露组相比,暴露低负荷组、暴露中负荷组PPAR-γ蛋白表达水平显著性升高(P<0.05);在PM 2.5暴露环境下,中等强度游泳训练可有效调节肝脏脂肪功能,抑制炎症状态,从而影响肝脏脂肪功能。
This work investigated the protein expression levels of moving rat liver under the exposure of PM 2.5 by using different loading swimming training modes.The result provides a theoretical basis for the effects of aerobic exercise on liver function in the exposed environment of PM 2.5.The rat training modes under the PM 2.5 exposure were set up as follow:PM 2.5 exposure section(E),low exposure section(LES),middle exposure section(MES)and high exposure section(HES).Elisa method was applied to detect the expression level of TNF-αand Western blot assay was used to evaluate the protein expression of PPAR-γ.The results showed that the TNF-αof MES was lower than that of other sections with the effective statistical significance at P<0.05.The PPAR-γexpression levels of LES and MES were significantly higher than that of E with the effective statistical significance at P<0.05.Therefore,under the exposure of PM 2.5,moderate-intensity training could effectively stimulate the cell factor and promote the signal deliver between Islet cell,as a result,the functions of the liver can be influenced.
作者
许磊
汪子铮
牛亦博
XU Lei;WANG Zizheng;NIU Yibo(Institute of Physical Education,Shanxi University of Finance&Economics,Taiyuan 030006,China;Sports Science Institute of Shanxi,Taiyuan 030012,China;China Railway Taiyuan Bureau Limited Company,Taiyuan 030009,China)
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第6期19-24,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
山西省体育局科研课题项目(17TY133)
山西省高校哲学社会科学项目资助(201803081)
山西省哲学社会科学规划课题资助(2019B159)
山西省教育科学十三五规划(GH-18032)
山西财经大学校级教学改革创新项目(2018246)
