摘要
目的:检测GATA6和骨形态发生蛋白(BMP2)在口腔鳞癌组织中表达情况,分析二者的表达关系并探讨其临床意义。方法:收集2014年4月至2016年12月经本院病理确诊的56例口腔鳞癌患者的石蜡标本作为观察组,同时取42例正常人的口腔粘膜组织作为对照组。采用免疫组化法测定口腔组织中GATA6、BMP2表达,分析其与口腔鳞癌临床病理特征的关系,并对全部患者进行随访,分析GATA6、BMP2表达对口腔鳞癌患者预后的影响。结果:口腔鳞癌组织中GATA6、BMP2阳性表达率均显著高于正常组织(P<0.05);GATA6、BMP2表达与口腔鳞癌患者TNM分期及肿瘤分化程度有关(P<0.05),与淋巴结转移、肿瘤大小无关(P>0.05);GATA6、BMP2高表达口腔鳞癌患者2年存活率均显著低于低表达患者(P<0.05);Cox模型多因素分析结果显示,TNM分期、肿瘤分化、GATA6、BMP2表达是影响患者预后的独立危险因素。结论:口腔鳞癌组织中GATA6、BMP2表达上调,与患者临床分期、肿瘤分化及2年生存情况有关,可能作为判断患者预后的生物标志物。
Objective:To detect the expressions of GATA6 and bone morphogenetic protein(BMP2)in oral squamous cell carcinoma(OSCC)tissues,analyze their relationship and explore their clinical significance.Methods:From April 2014 to December 2016,paraffin specimens from 56 patients with oral squamous cell carcinoma confirmed by pathology in our hospital were collected as observation group,and oral mucosa tissues from 42 normal persons were taken as control group.The expressions of GATA6 and BMP2 in oral tissues were detected by immunohistochemistry,and the relationship between GATA6 and BMP2 expression and clinicopathological characteristics of oral squamous cell carcinoma was analyzed.All patients were followed up and the prognostic effects of GATA6 and BMP2 expression on oral squamous cell carcinoma were analyzed.Results:The positive expression rates of GATA6 and BMP2 in oral squamous cell carcinoma tissues were significantly higher than those in normal tissues(P<0.05);the expressions of GATA6 and BMP2 were correlated with TNM stage and tumor differentiation in OSCC patients(P<0.05),but not with lymph node metastasis and tumor size(P>0.05);the 2-year survival rate of patients with high expressions of GATA6 and BMP2 in oral squamous cell carcinoma were significantly lower than those in patients with low expression(P<0.05);Cox model multivariate analysis showed that TNM stage,tumor differentiation,GATA6 and BMP2 expression were independent risk factors affecting the prognosis of patients.Conclusion:The expressions of GATA6 and BMP2 in oral squamous cell carcinoma are up-regulated,which is related to clinical stage,tumor differentiation and 2-year survival,it may be used as a biomarker for judging prognosis of patients.
作者
白真玉
刘德裕
陈尧卉
林道志
黄谢山
BAI Zhenyu;LIU Deyu;CHEN Yaohui(Haikou People's Hospital of Hainan/Haikou Hospital Affiliated to Xiangya Medical College of Central South University,Hainan Haikou 570208,China)
出处
《河北医学》
CAS
2019年第11期1872-1877,共6页
Hebei Medicine
基金
海南省医药卫生科研项目,(编号:1601320443A2001)
关键词
口腔鳞癌
GATA6
骨形态发生蛋白
Oral squamous
Cell carcinoma
GATA6
Bone morphogenetic protein
