摘要
目的分析香烟提取物对人支气管上皮细胞(HBE)抗衰老因子Klotho的影响及应对措施的效果。方法选择2016年3月至2017年8月在仪陇县人民医院进行体检的健康人员30例作为对照组,选择同期在该院治疗的具有10年吸烟史慢性阻塞性肺疾病(简称“慢阻肺”)病人30例作为观察组,对两组人群外周血细胞因子白细胞介素(IL) 8、IL 6、肿瘤坏死因子(TNF) α、Klotho蛋白表达水平进行评估。同时以香烟提取物刺激HBE细胞构建支气管炎症模型,并采用地塞米松进行干预。结果慢阻肺病人外周血IL 8,IL 6及TNF α含量均显著高于对照组,差异有统计学意义(t=3.012,P=0.013;t=4.092,P=0.000;t=3.421,P=0.008);慢阻肺病人外周血Klotho蛋白含量(0.00023±0.00003)pg/mL显著低于对照组(0.00842±0.00001)pg/mL,差异有统计学意义(t=4.112,P=0.000)。B组细胞培养上清液IL 8、IL 6、TNF α含量显著高于A组,差异有统计学意义(t=2.917,P=0.025;t=3.411,P=0.000;t=3.498,P=0.000)。B组细胞IL 8、IL 6、TNF αmRNA表达水平高于A组,均差异有统计学意义(t=2.802,P=0.027;t=3.212,P=0.000;t=3.092,P=0.000)。结论香烟提取物体外刺激HBE细胞,可显著降低Klotho因子含量,同时上调多种炎性因子基因的表达,体外给予地塞米松干预能够降低HBE细胞炎症反应。
objective to analyze the effect of cigarette extract on Klotho factor in HBE cells.Methods from March 2016 to August 2017,20 healthy persons in our hospital were selected as control group and 30 patients with chronic obstructive pulmonary disease(COPD)with 10 years of smoking history treated in our hospital as observation group.The expression of IL-8,IL-6,TNF-αand Klotho protein in peripheral blood was evaluated.At the same time,bronchitis model was established with cigarette extract to stimulate bronchial epithelial cells and treated with dexamethasone.Results The content of IL-8,IL-6 and TNF-αin patients with chronic obstructive pulmonary disease were significantly lower than those in the control group(t=3.012,P=0.013;t=4.092,P=0.000;t=3.421,P=0.008),and the content of Klotho protein in peripheral blood of patients with chronic obstructive pulmonarydisease was significantly lower than that of the control group[(0.00023±0.00003)pg/mL vs.(0.00842±0.00001)pg/mL,t=4.112,P=0.000].There was a significant difference(P<0.05)between group B and group B(P<0.05).The content of IL-8,IL-6,TNF-αin group B was significantly higher than that in group A(t=2.917,P=0.025;t=3.411,P=0.000;t=3.498,P=0.000).The expression level of the mRNA of IL-8,IL-6 and TNF-αin group B was higher than that in group A(t=2.802,P=0.027;t=3.212,P=0.000;t=3.092,P=0.000).Conclusion Cigarette extract can significantly reduce the content of Klotho factor and up-regulate the expression of gene of many inflammatory factors in HBE cells in vitro.Dexamethasone treatment in vitro can reducethe inflammatory response of HBE cells.
作者
杨林
贾钦尧
宋珊
YANG Lin;JIA Qinyao;SONG Shan(Department of Respiratory Medicine,Yilong People’s Hospital,Yilong,Sichuan 637600,China;Department of Respiratory Medicine,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)
出处
《安徽医药》
CAS
2019年第12期2450-2453,共4页
Anhui Medical and Pharmaceutical Journal
