摘要
目的探究苏叶黄连汤的体外抗流感作用与其HPLC指纹图谱间的相关性,明确苏叶黄连汤的药效物质基础。方法建立10批苏叶黄连汤的HPLC指纹图谱,采用MTT法考察其体外抗流感作用,采用主成分分析法构建二者的谱-效关系。结果建立了10批苏叶黄连汤的共有模式,相似度在0.997以上,共标定15个共有峰,与体外抗流感作用关系密切的6个共有峰分别为2、3、4、7、12和13号峰,其中3号和12号峰分别为迷迭香酸和盐酸小檗碱。结论第2、3、4、7、12和13号峰所代表的化合物可能是苏叶黄连汤发挥体外抗流感作用的主要药效成分。
Objective To explore the correlation between the anti-influenza effects of Suye Huanglian decoction and its HPLC fingerprints to study the active components with anti-influenza effects.Methods 10 batches fingerprints of Suye Huanglian decoction were established by HPLC,and to study the effects of anti-influenza virus by MTT.The correlation between areas of common peaks and anti-influenza virus in vitro were analysed by principle component analysis(PCA).Results The HPLC fingerprint method of 10 batches Suye Huanglian decoction was established,the similarity between the fingerprints of 10 batches of Suye Huanglian decoction were more than 0.997.Among which 15 peaks were common fingerprint peaks,the common peaks 2,3,4,7,12 and 13 were closely with the anti-influenza virus,among which the common peaks 3 and 12 were proved to be rosmarinic acid and berberine hydrochloride.Conclusion The 2,3,4,7,12 and 13 peaks are the principle anti-influenza substance in Suye Huanglian decoction.
作者
柳慧芸
吴巧凤
LIU Hui-yun;WU Qiao-feng(School of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 310053,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2019年第9期2049-2053,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(81274030
81473335)
浙江省自然科学基金(LY15H290003
LY18H280007)
关键词
苏叶黄连汤
指纹图谱
抗流感
谱效关系
Suye Huanglian decoction
Fingerprint
Anti-influenza
Spectrum-effect relationship
