摘要
[目的]探讨萝卜硫素对胃癌SGC-7901细胞增殖的影响及其对Wnt/β-连锁蛋白(Wnt/β-catenin)信号通路活化的影响。[方法]采用CCK8实验检测10、20、40μmol/L萝卜硫素对SGC-7901细胞增殖的影响,流式细胞仪分析萝卜硫素对SGC-7901细胞凋亡的影响,荧光素酶报告基因实验检测萝卜硫素对Wnt/β-catenin通路中淋巴增强因子/淋巴腺黏连因子(TCF4/LEF)转录活性的影响,Western Blot方法检测萝卜硫素对SGC-7901细胞中β-catenin、糖原合成激酶3β(GSK-3β)、c-Myc及半光天冬酶3(Caspase3)蛋白表达水平的影响。[结果]与对照组相比,萝卜硫素组SGC-7901细胞增殖抑制率显著升高(P<0.05),呈现出时间、剂量依赖性;萝卜硫素组SGC-7901细胞凋亡率相比对照组显著增高(P<0.05),并且萝卜硫素还能明显抑制TCF4/LEF报告质粒的荧光素酶活性(P<0.05);萝卜硫素组SGC-7901细胞内β-catenin及c-Myc蛋白表达呈浓度依赖性降低,而GSK-3β及Caspase3蛋白表达呈浓度依赖性增高(P<0.05)。[结论]萝卜硫素能明显抑制SGC-7901细胞增殖,并诱导其凋亡,其作用机制可能与抑制Wnt/β-catenin信号通路活化有关。
[Objective] To investigate the effect of sulforaphane on the proliferation of gastric cancer SGC-7901 cells and its effect on the activation of Wnt/β-catenin signaling pathway. [Methods] The effects of 10,20,40 μmol/L sulforaphane on the proliferation of SGC-7901 cells were detected by CCK8 assay. The effect of sulforaphane on the apoptosis of SGC-7901 cells was analyzed by flow cytometry.The luciferase reporter gene assay was used to detect the effect of sulforaphane on the transcriptional activity of lymphatic enhancement factor/lymphatic adhesion factor(TCF4/LEF) in Wnt/β-catenin pathway. Western Blot method was used to detect the effect of sulforaphane on the β-catenin,glycogen synthase kinase 3β,c-Myc and Caspase 3 protein expression levels in SGC-7901 cells. [Results]Compared with the control group,the inhibition rate of SGC-7901 cells in the sulforaphane group was significantly increased(P<0.05),showing a time-and dose-dependent manner;the apoptosis rate of SGC-7901 cells in the sulforaphane group was significantly higher than that in the control group(P<0.05),and sulforaphane could also significantly inhibit the luciferase activity of TCF4/LEF reporter plasmid(P <0.05). The expression of β-catenin and c-Myc protein in SGC-7901 cells of sulforaphane group was concentration-dependent increase,while the expression of GSK-3β and Caspase3 protein increased in a concentration-dependent manner(P<0.05). [Conclusion]The sulforaphane can significantly inhibit the proliferation and induce apoptosis of SGC-7901 cells,and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway activation.
作者
李丽
顾文燕
姜红
LI Li;GU Wenyan;JIANG Hong(Enshi Tujia and Miao Autonomous Prefecture Central Hospital Operating Room,Enshi 445000,China)
出处
《天津中医药》
CAS
2019年第11期1133-1136,共4页
Tianjin Journal of Traditional Chinese Medicine
