摘要
质量常数等级评价方法是一种将外观形态指标与内在含量指标相结合的中药材及饮片等级划分方法。该文将采用该方法进行栀子等级评价研究。通过测量14批不同栀子的形态指标及含量指标,建立果实类饮片的质量常数计算方法,形成等级评价标准。同时通过考察不同等级栀子样品对LPS诱导的RAW264. 7细胞NO抑制作用,考察饮片质量等级与药效之间的关系。结果显示所收集的栀子饮片质量常数范围为1. 46~4. 42;若以百分质量常数≥80%为一等饮片,50%~80%为二等饮片,剩余为三等饮片,则一等栀子饮片质量常数≥3. 54,二等栀子饮片质量常数应为2. 21~3. 54,三等栀子饮片质量常数<2. 21。药效结果显示,药效强度与等级正相关,证明了质量常数等级评价方法的合理性。
Grade evaluation method of quality constant is a kind of grading method for Chinese medicinal materials and decoction pieces,combining the external morphological index and internal content index. This method was used in this paper for grade evaluation of Gardeniae Fructus. By measuring the morphological and content indexes of 14 batches of Gardeniae Fructus,a method for calculating the quality constant of fruits was established,and the grade evaluation criteria were formed. At the same time,the NO inhibition effect of different grades of Gardeniae Fructus samples on RAW264. 7 cells induced by LPS was determined to investigate the relationship between the quality grade and pharmacodynamics of decoction pieces. The results showed that the quality constants of Gardeniae Fructus decoction piece samples ranged from 1. 46 to 4. 42. If the percentage quality constant ≥80% was classified into first-class,50%-80%as second-class and the rest as third-class,the quality constant was ≥3. 54 for first-class,2. 21-3. 54 for second-class and <2. 21 for third-class Gardeniae Fructus decoction pieces. The pharmacodynamic results showed that the pharmacodynamic intensity was positively correlated with the grade,which also proved the rationality of the grade evaluation method of quality constant.
作者
石佳
荆文光
邓哲
沈立
张雪婷
刘静
郭丛
陈畅
刘安
SHI Jia;JING Wen-guang;DENG Zhe;SHEN Li;ZHANG Xue-ting;LIU Jing;GUO Cong;CHEN Chang;LIU An(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Traditional Chinese Medicine Institute of China National Traditional Chinese Medicine Corporation,Beijing 102600,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第17期3732-3737,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81703945)
国家中医药管理局中药标准化项目(ZYBZH-Y-JX-27)
北京市科委“十病十药”研发专项(Z171100001717029)
关键词
栀子
质量常数
饮片等级
Gardeniae Fructus
quality constant
grades evaluation
