摘要
目的 :观察糖尿病早期膈肌酶组织化学变化 ,以了解糖尿病膈肌的损害。方法 :应用酶组织化学方法观察糖尿病 4周大鼠膈肌组织脱氢酶、水解酶和氧化酶活性变化。结果 :糖尿病 4周大鼠膈肌组织琥珀酸脱氢酶、苹果酸脱氢酶、异柠檬酸脱氢酶、谷氨酸脱氢酶、辅酶Ⅰ黄递酶、葡萄糖 - 6 -磷酸脱氢酶、酸性磷酸酶和酸性 -α-萘酸性酯酶活性强于对照组 ,乳酸脱氢酶和细胞色素氧化酶活性弱于对照组 ,辅酶Ⅱ黄递酶无明显改变 ,5’ -核苷酸酶不着色。图像分析显示糖尿病大鼠膈肌组织琥珀酸脱氢酶、苹果酸脱氢酶、异柠檬酸脱氢酶、谷氨酸脱氢酶、辅酶Ⅰ黄递酶、葡萄糖 - 6 -磷酸脱氢酶、酸性磷酸酶和酸性 -α -萘酸性酯酶着色深度 (A)明显高于对照组 ,而乳酸脱氢酶和细胞色素氧化酶明显低于对照组 ,辅酶Ⅱ黄递酶无明显差异。结论 :糖尿病 4周大鼠膈肌组织有氧氧化能力增强、糖酵解能力减弱、脂肪及能量代谢紊乱。
AIM: The aim of this research is to study the earlier enzyme activity changes of the diaphragm in diabetic rats. METHODS: An enzyme histochemical method was used to observe the changes in the enzyme activities of dehydrogenases,hydrolases and oxidases in 4th week diabetic rat diaphragm. RESULTS: The activites of enzymes including SDH(Succinate dehydrogenase),MDH(Malate dehydrogenase), GDH(Glutamate dehydrogenase), ICDH(Isocitrate dehydrogenase), NADHD(NADH diaphorase), G-6-PD(Glucose-6-phosphate dehydrogenase), ACP(Acid phosphatase) and ANAE(Acid α-naphtyl acid esterase) were increased in diabetic diaphragm compared with the control. LDH (Lactate dehydrogenase)and CCO(Cytochrome oxidase) activities were decreased, whereas NADPHD(NADPH diaphorase) showed no changes in diabetic rats. Eleven kinds of enzyme were analysed with image analysis.Optical density (A) of SDH, MDH, GDH, ICDH, NADHD, G-6-PD, ACP and ANAE in diaphragm of diabetic rats were significantly higher than that of control rats (P<0.01). A value of LDH and CCO in diaphragm from diabetic rats were significantly lower than that of control rats (P<0.01). A value of NADPHD in diaphragm from diabetic rats showed no apparent alteration compared with the control rats(P>0.05). CONCLUSION: Increase in the aerobic capacity, decrease in the glycolytic capacity, and disturbance of lipid and energy metabolism were found in diaphragm of 4th week diabetic rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第10期1276-1279,T006,共5页
Chinese Journal of Pathophysiology
关键词
糖尿病
实验性
膈
酶类
大鼠
Diabetes mellitus,experimental
Diaphragm
Enzymes
Rats
