摘要
目的 :探讨肾上腺髓质素 (AM)在慢性低氧性肺动脉高压发病中的作用。方法 :16只大鼠随机分为低氧组和对照组 ,间断常压低氧 14d复制肺动脉高压模型 ,静脉右心导管测定右室收缩压 (RVSP)的变化 ,分离心室检测右室 / (左室 +室间隔 ) (RV/LV +SP)比值 ,原位杂交法检测AMmRNA在右心室中的表达。结果 :低氧组大鼠右室压为 (6 3 6 3± 3 4 2 )mmHg ,显著高于对照组的 (34 13± 3 4 0 )mmHg(P <0 0 1)。低氧组大鼠 (RV/LV +SP)比值为0 4 39± 0 0 39,显著高于对照组的 0 2 3± 0 0 2 5 (P <0 0 1)。AMmRNA在对照组大鼠的右室心肌细胞也有少量表达 ,低氧组大鼠其表达显著多于对照组。图像分析表明 ,低氧组的平均吸光度、平均表达面积分别为 0 10 6 1± 0 0 188,0 14 2 1± 0 0 16 5 ,均显著高于对照组的 0 0 872± 0 0 171,0 0 96 7± 0 0 135。结论 :低氧时右心室AMmRNA表达增加 ,提示可能在肺动脉高压的发病中起保护作用。
AIM: To study the role of adrenomedullin(AM) in the pathogenesis of hypoxic pulmonary hypertension. METHODS: Rats were exposed to chronic hypoxia for 14 days. After the measurement of the right ventricular systolic pressure (RVSP), the rats were executed. The weight of the right ventricle (RV), the left ventricle(LV) and the ventricular septum(SP) were determined. The ration RV/(LV+SP) was used to express the thickness of RV. In situ hybridization was used for the detection of the expression of AM mRNA in the lung and RV. RESULTS: The RVSP in the hypoxic group was (63.63±3.42) mmHg,which was significantly higher than that in control group [(34.13±3.40) mmHg]. The RV/(LV+SP) in hypoxic group was 0.439±0.039,which was increased obviously when compared with that of control (0.230±0.025). The level of AM mRNA expressed in the RV in the hypoxia group was significantly higher than that in the control group. CONCLUSION: The expression of AM mRNA in RV increased in the hypoxic condition, which suggests that AM may attenuate the inappropriate increase in pulmonary artery pressure.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第10期1265-1267,T004,共4页
Chinese Journal of Pathophysiology
