摘要
目的 建立测定磷酸咯萘啶含量的HPLC方法。方法 选用DiamonsilC1 8(2 50mm× 4 .6mm ,5μm)色谱柱 ,0 .0 8mol·L- 1 磷酸二氢钾 -乙腈 (85∶1 5)为流动相 (含 0 .1 %三乙胺 ,pH3 .0 ) ;检测波长 2 78nm ;流速为 1 .0ml·ml- 1 ;进样量 2 0 μl。 结果 磷酸咯萘啶在浓度 5 .0 8～ 40 6 .4μg·ml- 1 范围内线性关系良好 ;回归方程为A =- 1 2 91 8+1 8657C ,r =0 .9999;平均回收率为 99.8% ,RSD =0 .8% (n =5)。结论 本法简便、灵敏。
OBJECTIVE To establish an HPLC method for the determination of malaridine phosphate. METHODS The HPLC system consisted of a Diamonsil C 18 column(250 mm×4.6 mm,5 μm) and 0.08 mol·L -1 potassium dihydrogen phosphate-acetonitrile (85∶15,containing 0.1% triethylamine,pH 3.0) as the mobile phase.The flow rate was 1.0 ml·min -1 and detection wavelength was 278 nm;RESULTS The linear range was 5.08～406.4 μg·ml -1 , r=0.9999; the average recovery was 99.8%, RSD=0.8%(n=5). CONCLUSION This method was simple, sensitive and accurate.
出处
《华西药学杂志》
CAS
CSCD
2002年第5期363-364,共2页
West China Journal of Pharmaceutical Sciences
