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小鼠卵巢巨噬细胞的免疫组化研究 被引量：1

Studies on Ovarian Macrophages in Mice by Immunohistochemical Method
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摘要 目的 :探讨卵巢内巨噬细胞在卵巢微细结构中的分布特征。方法 :用抗巨噬细胞表面的F4 / 80抗原的单克隆抗体对小鼠卵巢组织中巨噬细胞进行免疫组化染色后观察。结果 :巨噬细胞主要分布在卵巢的卵泡膜层、卵巢皮质和髓质的间质、闭锁卵泡和黄体组织中。排卵前卵巢表面包膜层出现大量的巨噬细胞 ,而妊娠期黄体中巨噬细胞骤减。结论 :巨噬细胞可能在不同的卵巢结构中对卵巢的生殖内分泌起不同的调节作用。 Objective: Our aim was to study the distributing character of ovarian macrophages in the micro structure of ovaries. Methods: Ovarian macrophages in mature mice were immunochemically studied by using a rat monoclonal antibody against macrophage specific antigen F4/80. Results: Macrophages mainly distributed in theca cell layer, ovarian cortex and interstitium of medullary, atretic follicle and corpus luteum. In pre ovulation phase, lots of macrophages appeared in ovarian capsule while the macrophages in pregnant corpus luteum reduced suddenly. Conclusion: It is probably that macrophages have different modulatary function on the reproductive endocrine in different micro structures of ovaries.
作者 李秀琴 银铎 伊藤正裕
机构地区 中国医科大学第二临床学院妇产科 日本香川医科大学第一解剖室
出处 《中国医科大学学报》 CAS CSCD 北大核心 2002年第5期352-353,358,共3页 Journal of China Medical University
关键词 卵巢 巨噬细胞 小鼠 ovary macrophage mice
分类号 R392.6 [医药卫生—免疫学]
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参考文献9

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同被引文献12

  • 1Bourguiba S, Chater S, Delalande C et al. Regulation of aromatase gene expression in purified germ ceils of adult male rats: effects of transforming growth factor beta, tumor necrosis factor alpha, and cyclic adenosine 3' 5'-monophosphate [J] . Biol Reprod, 2003, 69 (2): 592.
  • 2Kao YC, Higshiyama T, Sun X et al. Catalytic differences between porcine blastocyst and placental aromatase isozymes[J] . Eur J Biochem, 2000, 267 (20): 6134.
  • 3Ramadan AA , Selim SA , Hassan HM et al. Immune regulation of ovarian function in buffaloes (Bubalus bubalus) [J] . Theriogenology, 2001, 55 (2): 661.
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  • 5Marco MP , Martincz I , Amoedo ML. Vitamin D receptor genotype influences parathyroid hormone and calcitriol levels in predialysis patients [J] . Kit Int, 1999, 56 (4) : 1349.
  • 6Tsukamoto Y, Heishi M , Nagaba Yet al. More on hyperparathyroidism and vitamin D receptor [J] . Nature, 1996, 2 (11) : 1162.
  • 7Brannstrom M, Enskog A. Leukocyte networks and ovulation[J] . J Reprod Immunol, 2002, 57 (1 ~2): 47.
  • 8Kelly CC, Lyall H, Petrie JR et al. Low grade chronic inflammation in women with polycystic ovarian syndrome [ J] . Clin Endocrinol Metab, 2001, 86 (6): 2453.
  • 9Amato G, Conte M, Mazziotti Get al. Serum and follicular fluid cytokines in polycystic ovary syndrome during stimulated cycles [J] . Obstet Gynecol, 2003, 101 (6) : 1177.
  • 10Duan I, Gan H, Arm Jet al. Cytosolic phospholipase A2 participates with TNF - alpha in the induction of apoptosis of human macrophages infected with mycobacterium tuberculosis H37Ra [ J] . J lmmunol, 2001, 166 (12): 7469.

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中国医科大学学报
2002年 第5期

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