摘要
目的制备一种以胶原为基质的组织工程支架材料,并研究其血液相容性及组织相容性。方法采用物理、化学及物理/化学方法对胶原材料进行交联。结果动态凝血试验显示,通过不同方法交联后的胶原材料,D(λ)值随着与血液接触时间的延长而下降,其中,通过碳化二亚胺交联得到的胶原在与血液接触相同时间后测得的D(λ)值最高;溶血率的数据表明,交联后的胶原材料溶血率均小于5%;血小板粘附与变形电镜照片显示,血液在3种材料表面均未引起血小板的变形,且粘附数量较少。结论3种交联法制备的胶原材料都有较好的血液相容性,材料表面细胞生长状况良好,表明材料具有良好的组织相容性。
Objective To prepare a tissue-engineered scaffold material using collagen as the matrices and to study the blood compatibility and tissue biocompatibility of this material. Methods Physical, chemical and physical/chemical methods were used for the crosslinking of the collagen. Results Dynamic blood clotting tests indicated that the blood clotting index (BCI) of the crosslinked collagen materials prepared by different means decreased as their contact with the blood was prolonged, and the collagen material obtained after crosslink through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method showed the highest BCI after contact with the blood within certain length of time. Hemolysis ratios of all the crosslinked collagen materials were shown to be much lower than 5%, well conforming to the requirement of biomaterials. Scanning electron microscopy showed that the platelets attached to the surface of the crosslinked collagen materials, having a fairly small number, were not morphologically distorted. Conclusion The collagen materials obtained by the 3 crosslink methods have good blood compatibility. The cells grow well on the surfaces of the materials, indicating their good biocompatibility.
出处
《第一军医大学学报》
CAS
CSCD
北大核心
2002年第10期878-879,882,共3页
Journal of First Military Medical University
基金
国家科技部863项目(2001AA625050)
关键词
胶原
交联试剂
血液相容性
组织相容性
collagen
cross-linked reagent
blood compatibility
histoompatibility
