摘要
1987年首次报道,在塞内加尔存在血清乙型肝炎病毒表面抗原(HBsAg)阳性,但核心抗体(anti-HBc)阴性的感染者。其血清中有直径为45～60nm的病毒,也有22～30nm直径的HBsAg颗粒。与常规乙型肝炎病毒(HBV)DNA探针进行杂交,15份感染者中仅有一份出现杂交微弱阳性,当时被称为是乙型肝炎病毒2型(HBV-2)。此后,法国、
To study the core gene of Hepatitis B virus type 2 strain, an HBsAg positive but anti-core, anti-e and anti-HBs negative serum ( #61) was used to extract DNA. Primers were used to amplify partial Pre-core and core gene by polymerase chain reaction. The amplified product was cloned in vector pGEM 3Z, and a clone(CL 61-8)was further sequenced. By comparing the sequence to that of a preiously cloned conventional HBV DNA sequence(pADR-l),only 4 nucleotide variations were found. However, all variations did not lead to alterations in amino acid sequence. Our data do not support the former presumption that there are variations in the core gene and core protein in HBV-2, which is responsible for non-detectable anti-HBc.Therefore it is hi.ghly possible that lack of anti-HBc is due to defect in host immune responses, rather than variations in the virus perse.Complete immunetolerance versus HBc Ag may be the cause of non-detectable anti-HBc antibody.
出处
《病毒学报》
CAS
CSCD
北大核心
1992年第1期90-92,共3页
Chinese Journal of Virology
关键词
乙肝病毒
核心基因
核心抗体
Hepatitis B virus type 2 Nucleotide sequenceHepatitis B virus core gene
