摘要
目的 检测人 β -NGF基因转染CHO细胞后培养上清中所表达的 β -NGF的生物学活性。 方法 采用脂质体介导的真核细胞转染 ,运用MTT法进行检测分析。结果 转染 β-NGF基因的CHO细胞可分泌能促进PC12细胞生长的活性 β -NGF。 结论 转染 β -NGF基因的CHO细胞所分泌的 β -NGF接近自然合成的蛋白 ,具有较高的生物学活性 ,为NGF的生物制药奠定了实验基础。
Objective To detect the biological activity of the expression products of the human β-NGF cDNA in CHO cell line.Methods Human β-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by the method of MTT.Results The protein in the culture supernatent of the positive CHO cells transfected with β-NGF gene could stimulate the growth of PC12 cell line.Conclusion The target gene expressed successfully in the transfected CHO cell line and had good biological activity.
出处
《苏州大学学报（医学版）》
CAS
2002年第5期501-502,509,共3页
Suzhou University Journal of Medical Science
基金
江苏省科委应用基金资助项目 (BJ990 70 )
