摘要
目的 检测献血员血液中巨细胞病毒DNA ,了解献血员感染巨细胞病毒的情况 ,防止由于输血而发生医源性感染。方法 采用快速、敏感、特异的聚合酶链反应 (PCR)电泳法及PCR ELISA法对 2 12例献血员进行人类巨细胞病毒 (HCMV)DNA的检测。结果 18例HCMV DNA阳性 ,占被检对象的 8 4 9% (18/ 2 12 ) ;同时显示HCMV DNA阳性率与献血员性别、年龄段差异无显著性相关。结论 由于献血者中存在着HCMV感染的情况 ,故在临床治疗用血时 ,应对献血者进行HCMV的检测 。
Objective To detect the levels of serum HCMV DNA in blood donor to avoid the HCMV infection caused by transfusion. Methods Two hundred and twelve blood donors were studied for HCMV DNA by using PCR electrophoresis and PCR ELISA. Results Serum HCMV DNA levels of eighteen blood donors were positive, which composed of 8.45%. Furthermore, the positive rate of HCMV DNA in blood donors was not correlated with the sex and ag e respectively. Conclusion Because of the HCMV infection in blood donor, it is necessary to detect the HCMV DNA in blood donors to assure the safety of blood receptors.
出处
《中国实验诊断学》
2002年第5期333-334,共2页
Chinese Journal of Laboratory Diagnosis
