摘要
目的:探讨人脐血造血干细胞宫内移植的更好途径。方法:将人脐血单个核细胞注入大鼠胚胎的卵黄囊及胎鼠腹腔内,并设阴性及空白对照组,观察手术并发症及妊娠结局,待出生后1个月及2个月,分别用流式细胞仪和免疫组化检测移植情况。结果:胎鼠腹腔途径组的手术并发症发生率、胎鼠丢失率显著高于卵黄囊途径组(P<0.001,P<0.05)。出生后实验组仔鼠外周血中检测到逐渐增加的人CD3细胞,卵黄囊组的增殖量高于腹腔途径组(P<0.05)。卵黄囊组的种植率为89.7％,腹腔组的种植率为64.0％,二者比较有显著性差异(P<0.05)。出生后2个月在仔鼠的肝、脾及胸腺组织中均检测到人CD3、CD20及CD34^+阳性细胞,卵黄囊组的表达量明显高于腹腔组(P<0.001)。结论:大鼠胚胎的卵黄囊途径是人脐血造血干细胞进行宫内移植的一条较好途径。
Objective:we used human cord blood hematopoietic stem cells in utero transplantation through yolk sac and fetal abdominal cavity of rats, and compare the complications and the engraftment rate. Methods: Human cord blood (MNC) cells were transplanted into the yolk sac and fetal abdominal cavity of rats, and the complications and the outcome of pregnancy were observed. The condition of engraftment was detected with flow cytometry assay and immunohistochemistry methods after the fetus were born. Results:The incidence of complication of group fetal abdominal cavity was higher than that of group yolk sac (P< 0. 001). The fetal loss rates of group fetal abdominal cavity were higher than that of group yolk sac (P< 0. 05). Human CD3 cells were detected in rats and the engraftment rate were 89. 7% and 64. 0% .respectively. There were significant difference between them (P<0. 05). Human CD3,CD20 and CD34+ cells were also determined in liver,spleen and thymus of rats at 2 months of age. Conclusion: Human cord blood cells transfused into the yolk sac and fetal abdominal cavity were engrafted. Some complications occurred during operations which affected the outcome of pregnancy. Yolk sac is a better way for in utero transplantation.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2002年第5期267-272,301,共7页
Reproduction and Contraception
基金
广东省科委攻关基金资助(资助号9827821)
关键词
大鼠
胚胎
卵黄囊
腹腔
人脐血
造血干细胞移植
hematopoietic stem cells
fetal blood
in utero
transplantation
yolk sac
