摘要
目的 :建立RP HPLC法测定晕复静片中士的宁含量的分析方法。方法 :采用反相高效液相色谱法 ,使用Kromasil C18色谱柱 (5 μm ,15 0mm× 4 .6mm) ,以水 甲醇 乙酸 三乙胺 (2 0 0∶10 0∶2 .0∶0 .3)为流动相 ,流速 1.0ml/min ,检测波长为 2 5 4nm。结果 :本方法线性关系良好 ,线性范围0 .172 8～ 0 .86 40 μg (r =0 .9994 ) ,士的宁的平均加样回收率为 99.3% ,RSD =0 .5 %。结论 :本方法操作简便 ,分离效果好 ,结果准确 ,可用于晕复静片的质量控制。
Objective:To establish the method for assaying the content of strychnine in Yunfujing tablets. Method: The reversed phase-high performance liquid chromatography with Kromasil-C_ 18 column(5 μm,150 mm×4.6 mm) was used . Water-methanol-acetic acid-thiethylamine (200∶100∶2.0∶0.3) was used as mobile phase. The flow rate was 1.0 ml/min,and the detection wavelength was at 254 nm. Result: Strychnine over the range of 0.172 8～0.864 0 μg had a good linear relation(r=0.999 4) .The average recovery was 99.3% with RSD 0.5%(n=5).Conclusion: The method is convenient for a good resolution and can be used for the quality control of Yunfujing tablets.
出处
《安徽中医学院学报》
CAS
2002年第6期52-53,共2页
Journal of Anhui Traditional Chinese Medical College
关键词
RP-HPLC法
测定
晕复静片
士的宁
反相高铲液相色谱法
中药
Yunfujing tablets
Content determination
Strychnine
Reversed phase-high performance liquid chromatography
