摘要
目的 :构建截短型的髓性造血细胞抑制因子 (myeloid progenitor inhibitory factor 1 ,MPIF-1 )表达载体 ,并在大肠杆菌中进行高效表达。方法 :利用 PCR方法从 p Kp L3 a-MPIF-1的表达载体上扩增 MPIF-1(2 5 -99)基因片段 ,重组到 p MTY4载体 ,并在大肠杆菌 pop2 1 3 6中高效表达。所得包涵体经过变性、透析复性后 ,用阴离子交换层析柱纯化。结果 :获得了 MPIF-1 (2 5 -99)的高效表达 ,表达量约占菌体总蛋白的 3 0 %。结论 :在大肠杆菌高效表达 MPIF-1 (2 5 -99)融合蛋白。
Objective: To construct the expression vector of the truncated form of myeloid progenitor inhibitory factor 1(MPIF-1) and express in E.coli. Methods: The gene encoding C terminal 74 amino acid fragment of MPIF-1 was inserted into expression vector pKpL3a and pMTY4 and transformed E.coli host strain pop2136. Results: The results of SDS-PAGE electrophoresis demonstrated that the temperature induced bacteria expressed an extra band of 20kD in comparison with the uninduced ones. The expression level was more than 30 % of the total cell lysate. Conclusion: The recombinant MS2/MPIF-1(25-99) was efficiently expressed in E.coli.\;
出处
《内蒙古医学院学报》
2002年第3期153-155,167,共4页
Acta Academiae Medicinae Neimongol
