摘要
为筛选抗IBDV方剂的主药,采用组织培养方法,测试了黄芪、连翘等8种中药粗提物在体外鸡胚成纤维细胞(CEF)上的最大安全浓度及其对CEF增殖、存活时间的影响。结果显示,黄芪对CEF基本无毒性作用,最大安全浓度为25mg/ml,而连翘等其余7种中药粗提物则表现出较大的毒性作用,最大安全浓度极低,同时,黄芪对CEF还具有较强的促生长和延长存活时间的作用。因此,筛定黄芪作为抗IBDV方剂的主药。
To screen the principals of the prescriptions resistant to infectious bursal disease virus (IBDV),the maximum safety concentration of extracts of 8 Chinese herbal medicines such as radix astragali,fructus forsythiae,et al on chick embryo fibroblast(CEF) multiplication and survival time were studied by tissue culture.Radix astragali showed little toxicity to CEF,the maximum safety concentration being 25 mg/ml.But other 7 Chinese herbal medicines were extremely poisonous,the maximum safety concentration being quite low.Radix astragali could promote reproduction and prolong lifetime of chick embryo fibroblast.For this reason, radix astragali was chosen as the principal in antiIBDV prescriptions.
出处
《江苏农业学报》
CSCD
2002年第4期228-232,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省畜禽生物制品工程技术研究中心开放课题(ZY118)
关键词
中药
粗提物
鸡胚
成纤维细胞
生长
安全浓度
Chinese herbal medicine
extract
chick embryo fibroblast
maximum safety concentration
