摘要
本研究探讨三氧化二砷 (As2 O3)对两种慢性粒细胞白血病 (CML)细胞系有无作用及作用机制。实验选用了两种不同的CML细胞系KU81 2和MEG 0 1及两种其它白血病细胞系U937和PL2 1 ,加入不同浓度的As2 O3(0 .2 ,2和 1 0 μmol/L) ,在不同时间计数活细胞数 ,观察细胞形态 ,并以TUNEL法和DNA凝胶电泳检测细胞有无凋亡的发生 ;同时检测 0 .2 μmol/LAs2 O3作用时细胞表面CD34 ,CD1 3 ,CD33 ,CD1 9,CD1 1b ,CD1 4和CD7的变化 ,并通过RT PCR检测细胞bcr abl水平及caspase 3的变化。研究结果发现 ,不同浓度的As2 O3对 4种细胞系的作用不同 ,1 0 μmol/L时 4种细胞系均会发生细胞凋亡 ,2 μmol/L时则仅CML细胞系KU81 2和MEG 0 1会产生凋亡 ,0 .2μmol/L时 4种细胞系均不发生凋亡。在培养 2 4小时后 ,4种细胞系表面的CD34 ,CD1 3 ,CD33 ,CD1 9,CD1 1b ,CD1 4和CD7等均无明显改变。RT PCR检测bcr abl的水平发现在 2种CML细胞系中无明显改变 ,其caspase 3的活性较对照细胞均有所增加。结论 :As2 O3在较高浓度下可诱导 4种白血病细胞系产生凋亡 ,在 2 μmol/L仅可诱导CML来源的两种细胞系产生凋亡 ,CML细胞系对As2 O3较其它两种细胞系敏感 ,其作用机制与caspase
The objective is to explore the effect and the mechanism of arsenic trioxide(As 2O 3) on different cell lines of chronic myeloid leukemia(CML). Different concentrations of As 2O 3 (0.2, 2 and 10 μmol/L) were added to CML cell lines KU812 and MEG 01 and other leukemia cell lines U937 and PL21, the cell numbers were counted at different times, TUNEL and DNA ladder were assayed. Different antibodies, CD34, CD13, CD33, CD19, CD11b, CD14 and CD7, were added to detect the change of the molecules on cell surface, the change of bcr abl by RT PCR and the activity of caspase 3 were assayed. The results showed that different concentrations of As 2O 3 had different effects on the survival of the 4 cell lines. After culture for 24 hours with As 2O 3, there was no significant increase in CD11b in all the four cell lines. There were no changes of bcr abl in the 2 CML cell lines treated and untreated with As 2O 3 by RT PCR. Activities of caspase 3 were all increased. It is concluded that As 2O 3 can induce apoptosis in CML cell lines, the concentration to induce apoptosis is different, CML cell lines are more sensitive than the other 2 leukemia cell lines. As 2O 3 induced apoptosis may have some relation with the activation of caspase 3.
出处
《中国实验血液学杂志》
CAS
CSCD
2002年第5期413-418,共6页
Journal of Experimental Hematology
