摘要
利用凝胶色谱、摩尔比法及紫外光谱确定了铬(Ⅲ)与人血清白蛋白(HSA)的结合作用。HSA上存在两类铬(Ⅲ)结合部位,比较强的结合部位数为3。电荷转移谱的计算表明,HSA的强结合部位的配位原子是氮和氧,它们与铬(Ⅲ)形成八面体结构。
The binding of chromium (III) to buman scrum albumin (HSA) and the stoichiometric relationhave been confirmed by gel chromatography, mole-ratio method and ultroviolet spectrum. HSA hastwo groups of binding sites to Cr(III) and the number of strong binding sites is 3. Comparisonof the estimated values and the experimental charge transfer spectrum data indicate that, the Cr(III)-HSA complex bas an octahedral configuration, and the ligating atoms are nitrogen and oxygen.
出处
《北京医科大学学报》
CSCD
1992年第3期219-221,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
人
血清白蛋白
铬
电荷转移谱
Human serum albumin
Chromium
Charge transfer spectrum
