摘要
目的 鉴定日本血吸虫成虫肠道内容物中的金属蛋白酶。 方法 以明胶作底物 ,利用明胶 SDS PAGE分离日本血吸虫成虫肠道内容物 ,将电泳后的凝胶于不同 pH缓冲液和酶抑制剂中进行孵育 ,对其中的金属蛋白酶进行分析和鉴定。 结果 日本血吸虫成虫肠道内容物中存在降解明胶的金属蛋白酶 ,其最适 pH值为 7～ 9。金属蛋白酶抑制剂EDTA抑制其活性。电泳分离获得有活性的酶蛋白 ,该酶是血吸虫感染血清识别的弱抗原。
Objective To identify the proteinases in intestinal contents of Schistosoma japonicum (S.japonicum) adult worms. Methods Proteinases were fractionated through gelatin SDS PAGE, and gelatin was degraded by proteinases when the gel was incubated in buffer with optimal pH. Metalloproteinase which degrades gelatin was identified by incubating gel in optional buffer containing different enzyme inhibitors. Metalloproteinase was isolated through SDS PAGE. Results A metalloproteinase was identified in intestinal contents of S.japonicum adult worms. The optimal pH of metalloproteinase is pH 7-9. The activity of the metalloproteinase was inhibited by EDTA. The metalloproteinase showed enzymatic activity after being fractionated through SDS PAGE and weakly reacted with sera of rabbits infected with S.japonicum . Conclusion A metalloproteinase is present in intestinal contents of S.japonicum adult worms.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2002年第5期298-301,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
湖南省科技厅资助 (No .0 0JZY2 1 1 5)
世界卫生组织热带病研究培训专门规划资助项目 (No .980 2 68)~~
关键词
鉴定
日本血吸虫
成虫
肠道内容物
明胶
电泳
抑制剂
金属蛋白酶
Schistosoma japonicum ,adult worm,intestinal contents,gelatin, electrophoresis,inhibitor,metalloproteinase
