摘要
目的 观察临床常用的免疫抑制剂对急性胰腺炎时炎症相关性细胞因子紊乱的调节作用 ,并探讨免疫抑制剂治疗胰腺炎的效果和机理。方法 实验用雄性SD大鼠 3 8只 ,共分 6组 :正常对照组 (n =6) ;胰腺炎组 (n =8) ,采用开腹胰管注射 5 %牛磺胆酸钠制备急性胰腺炎动物模型 ;余下 4组 (均为n =6)在胰腺炎诱导成功后 0 .5h按静脉注射药物不同分为 :5 Fu治疗组、甲基强的松龙治疗组、环磷酰胺治疗组及氨甲碟呤治疗组。手术后 2 4h处死动物 ,分别检查血中TNFα、IL 1、IL 6、IL 10、TGF β及胰淀粉酶和胰腺湿重。 结果 在急性胰腺炎时 ,动物血中的炎症性细胞因子和抗炎症细胞因子均显著增高 ,在使用免疫抑制剂治疗后 ,上述细胞因子均有不同程度下降。其血胰淀粉酶和胰腺湿重也明显好转。结论 免疫抑制剂可以抑制急性胰腺炎时异常增高的炎症细胞因子和抗炎症细胞因子。通过矫正免疫异常的方式缓解胰腺炎所致的病理生理紊乱 ,达到治疗急性胰腺炎的目的。
Objective To observe the effecacy of immunosuppressive agents on modulation of the disorders of inflammatory and anti inflammatory cytokines in acute pancreatitis, and to investigate the mechanism of treatment of acute pancreatitis with immunosuppressive agents. Methods SD male rats were divided into 6 groups: group 1, the normal control group ( n =6); group 2, acute pancreatitis induced by ductual injection of 5%sodium cholate sulfur at the volume of 1.0 ml/kg without treatment ( n =8). After the pancreatitis were induced, the rest rats were injected intravenously with 5 Fu 40 mg/kg (group 3, n =6); or methylprednisolone 30 mg/kg (group 4, n =6); or cyclophosphamide 20 mg/kg (group 5, n =6); or methotrexate 1.2 mg/kg (group 6, n =6). Twenty four hours after operation, the animals were killed, the blood samples were taken for measurement of TNFα, IL 1, IL 6 (by bioassay), and IL 10, TGF β (by ELISA) as well as amylase. Results The inflammatory cytokines (TNFα,IL 1,IL 6 ) and the anti inflammatory cytokines (IL 10 and TGFβ), in blood of acute pancreatitis were increased significantly. After treated with immunosuppressive agents, both the inflammatory and anti inflammatory cytokines were decreased in different degrees. Some indexes of the severity of acute pancreatitis, such as amylase and pancreatic weight were improved obviously.Conclusion Immunosuppressive agents can regulate inflammatory associated cytokines increased remarkably in the acute pancreatitis. Therefore, improvement of acute pancreatitis can be achieved through rectifying the abnormal immunity and relieving the pathophysiological disorders of the acute pancreatitis by immunosuppressive agents.
出处
《中国普外基础与临床杂志》
CAS
2002年第6期384-387,共4页
Chinese Journal of Bases and Clinics In General Surgery
