摘要
研究了有机酸根和磷酸根等配体体系中 ,针铁矿、高岭石和黄棕壤、砖红壤胶体对酸性磷酸酶的吸附。结果表明 ,磷酸根对针铁矿表面酶的吸附量抑制最显著 ,酒石酸根对酶与土壤胶体和高岭石表面酶的吸附量影响最强。随着配体浓度增加 ,乙酸根对酶吸附的影响表现为先促进 (0～ 10mmol·L-1)后轻微抑制 (>10mmol·L-1) ;草酸则表现为先抑制 (0～ 5mmol·L-1)后促进 (5～ 5 0mmol·L-1) ;酒石酸和磷酸对酶的吸附表现为一直抑制。在乙酸、草酸和低浓度的酒石酸和磷酸根体系中 ,酶在土壤胶体和矿物表面的吸附等温线符合Langmuir方程 (L型 ) ,高浓度的酒石酸和磷酸对应的吸附等温线符合线性方程 (C型 )。在酒石酸根和磷酸根体系中 ,配体与酶加入顺序对酶吸附的影响较大 ,一般来说 ,配体和酶同时进入体系具有最低的酶吸附量。
Adsorption of acid phosphatase on goethite, kaolinite, yellow brown soil and latosol colloids in the presence of organic acids and phosphate was studied. The results showed that the inhibitory effect of phosphate on enzyme adsorption of goethite was the most remarkable. However, for soil colloids and clay minerals, the inhibitory effect of tartrate was the greatest on enzyme adsorption. With the increase of ligand concentration, acetate showed stimulative effect on enzyme adsorption in 0-10mmol·L -1 solutions. But the amount of enzyme adsorption decreased when acetate concentration was higher than 10mmol·L -1 . In 0-5mmol·L -1 oxalate solutions, the amount of enzyme adsorbed decreased quickly, followed by an increase of enzyme adsorption. Tartrate and phosphate showed durative inhibitory effect on enzyme adsorption in concentrations studied. In acetate, oxalate and lower concentration of tartrate and phosphate solutions, the adsorption isotherms of enzyme conformed to the Langmuir equation (L type). When the concentration of tartrate and phosphate was higher, the adsorption isotherms of enzyme conformed to the linear equation (C type). The influence of the addition order of ligand and enzyme on enzyme adsorption was greater in tartrate and phosphate systems. In general, simultaneous introduction of ligand and enzyme into system had the lowest amount of enzyme adsorption.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第11期1375-1380,共6页
Scientia Agricultura Sinica
基金
教育部优秀青年教师资助计划
