摘要
目的:研究单侧喉返神经(RLN)切断后,两侧甲杓肌纤维所发生的酶组织化学变化。方法:建立单侧RLN麻痹动物模型,应用琥珀酸脱氢酶染色法及显微图像分析仪,动态观察双侧甲杓肌中各型肌纤维直径的变化。结果:去RLN侧4周时各型肌纤维萎缩有意义(P<0.05);10周时,已分辨不出肌型,肌纤维呈纤溶性改变。健侧甲杓肌,4周时直径已发生代偿改变,各型肌纤维均增粗(P<0.05);10周时代偿尤为显著,其中以中间肌纤维代偿最为明显,各型肌纤维构成比没有明显变化趋势。结论:当发生单侧RLN麻痹时,健侧甲杓肌各型肌纤维均增粗,以中间肌纤维最明显,对喉的功能起到一定的代偿作用。
Objective: To chronologically evaluate the changes in both sides of thyroarytenoid (TA) muscles when one side of recurrent laryngeal nerve (RLN) was cut off. Method:In 7 adult mongrel dogs, 2 dogs for control and the other 5 dogs for the following experiment: a 2 cm segment of right RLN was excised under intraper-itoneal sedation. The animals were killed at 2 ,4 ,6 ,8 ,10 week respectively and the thyroarytenoid were processed for histochemical reaction and electromicroscope. The mean muscle fiber, standard deviation and muscle fiber type composition were determined. Result:For the injured sides, denervation resulted in shrinking of all the fibers at four weeks. At ten weeks , however , all the muscles were fibrosed . For the uninjured sides (left side) , TA diameter appeared compensatory changes at four weeks, this changes were obvious at ten weeks. Variability of the fiber composition was observed throughout the study; however,there was no specific trend in changes before ten weeks. Conclusion: When the RLN was excised, the fibers in the uninjured side of thyroarytenoid appeared compensatory changes in fiber diameter, which could give rise to the recovery of the laryngeal function.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2002年第11期607-609,共3页
Journal of Clinical Otorhinolaryngology
