摘要
A solid-phase-radioimmunoassay (SPRIA) for the monitoring of blood digoxinlevel has been developed, in which a secondary antibody-coated polystyrene tubes are used.This noval method seems to be simple to use and only takes about an half hour. The standardcurve is linear from 0.25 to 4 μg/L. The sensitivity of the detection is 0.1 μg/L. Reproducibilitystudies with 3 control sera of 0.5-2.5 μg/L give illtraassay CV< 5% and interassay CV< 10%.The specimens are measured and compared with those of the conventional radioimmunoassayand the values are well correlated (r=0.96, Y=1.022X+0.04 μg/L).
A solid-phase-radioimmunoassay (SPRIA) for the monitoring of blood digoxinlevel has been developed, in which a secondary antibody-coated polystyrene tubes are used.This noval method seems to be simple to use and only takes about an half hour. The standardcurve is linear from 0.25 to 4 μg/L. The sensitivity of the detection is 0.1 μg/L. Reproducibilitystudies with 3 control sera of 0.5-2.5 μg/L give illtraassay CV< 5% and interassay CV< 10%.The specimens are measured and compared with those of the conventional radioimmunoassayand the values are well correlated (r=0.96, Y=1.022X+0.04 μg/L).
