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藻红蓝蛋白裂合异构酶对几种脱辅基藻胆蛋白的催化作用 被引量：2

Catalysis of Lyase-isomerase PecE/PecF for Several Apophycobiliproteins
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摘要 PecE PecF是层理鞭枝藻藻红蓝蛋白α亚基 (α PEC)生物合成的裂合异构酶。以 4种脱辅基藻胆蛋白为底物 ,初步研究了PecE PecF对底物蛋白的催化专一性。结果表明 ,PecE PecF可催化藻蓝胆素 (PCB)与高度同源的层理鞭枝藻不同亚种的α PEC脱辅基蛋白的体外重组 ,也可催化经 12 8位Trp定点突变到Phe而得到的α PEC脱辅基蛋白的体外重组 ,但PecE PecF对PCB与藻蓝蛋白α亚基 (α CPC)脱辅基蛋白的体外重组无催化作用。α PEC脱辅基蛋白的重组不受表面活性剂TritonX 10 0的影响 ,而TritonX 10 0可改进PCB与α Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC α-subunit(α-PEC).In this work,the specificity of PecE/PecF on substrate apoproteins was reported.PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of α-PEC from two different subspecies of Mastigocladus laminosus,as well the site-directed mutated apoprotein of α-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin α-subunit(α-CPC) from Mastigocladus laminosus.The surfactant Triton X-100 had no effect for the reconstitution of α-PEC,while it could improve the reconstitution of PCB with apoprotein of α-CPC.
作者 朱菁萍 周明 赵开弘 曾志雄 周宜开
机构地区 华中科技大学同济医学院公共卫生学院 华中科技大学环境科学与工程学院
出处 《生物工程学报》 CAS CSCD 北大核心 2002年第6期703-708,共6页 Chinese Journal of Biotechnology
基金 国家自然科学基金资助 (No .30 2 70 32 6 )~~
关键词 藻红蓝蛋白裂合异构酶 脱辅基藻胆蛋白 催化作用 lyase-isomerase, phycobiliprotein, reconstitution
分类号 Q55 [生物学—生物化学]
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参考文献7

  • 1Zhao K H,Deng M G,Zhen M et al. Novel activity of a phycobiliprotein lyase:both the attachment of phycocyanobilin and the isomerizat ion to phycoviobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin.FEBS Letters,2000,469:9～13
  • 2Storf M,Parbel A,Meyer M et al. Chromophore attachment to bi liproteins:specificity of PecE/PecF,a lyase-isomerase for the photoactive 31-Cys-α84-phycoviolobilin chromophore of phycoerythrocyanin.Biochemistry,20 00,40:12444～12456
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同被引文献11

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生物工程学报
2002年 第6期

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