摘要
目的 :建立牛黄肛炎宁栓的含量测定方法。方法 :采用高效液相色谱法测定体外培育牛黄中胆红素的含量 ;采用双波长薄层扫描法测定黄连、黄柏中盐酸小檗碱的含量。结果 :胆红素在 0 .0 2 8～ 0 .450 g·L- 1内呈良好的线性关系 (r =0 .9999) ,平均回收率为 97.9% (RSD =1.7% ,n =5)。盐酸小檗碱在 0 .188～ 1.12 8μg内呈良好的线性关系 (r =0 .999) ,平均回收率为 97.4% (RSD =2 .9% ,n =5)结论 :本法准确可靠 ,简便易行 ,可用于本品及含体外培育牛黄、黄连、黄柏等相关制剂的质量控制。
OBJECTIVE To establish a quality control method for Niuhuang Gangyan Ning suppositories. METHODS RP HPLC was used to determine the content of bilirubin and dual wavelength flying spot zigzag scannining was used to determine the content of berberine in the suppositories. RESULTS The liner range of bilirubin was 0.028 ～ 0.450 g·L -1 ( r = 0.9999 ) while the average recovery was 97.9% (RSD= 1.7% , n =5).The line range of berberine was 0.188 ～ 1.128 μg( r = 0.999 ) and the average recovery was 97.4% (RSD= 2.9% , n =5).CONCLUSIONS The methods is sutiable for quality control with good chromatographic separation.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第11期658-661,共4页
Chinese Journal of Hospital Pharmacy
