摘要
本法在去除蛋白与核酸的条件下以 2 5 4nm作为脂多糖 (Lipopolysaccharide ,LPS)凝胶色谱检出波长 ,得到较高纯度志贺菌LPS。LPS经乙酸水解后 ,仍以 2 5 4nm作为O抗原多糖 (O -polysaccharide ,OPS)凝胶色谱的检出波长 ,可获得纯度优于常规方法且具较好免疫反应性的OPS ,该OPS与牛血清白蛋白 (bovineserumalbumin ,BSA)结合后具有较好免疫原性。
We prepared the purified lipopolysaccharide(LPS)from Shigella flexneri 5 M90T by enzymatic hydrolysis and Sephadex G50 chromatography Subsequently the O polysaccharide(OPS)was isolated from the LPS by acetic acid hydrolysis,and was purified by gel chromatography with 254nm ultraviolet monitor The OPS reacted with the antiserum to M90T The OPS BSA conjugate efficiently induce specific antibodies against whole M90T bacterial cell
出处
《中国人兽共患病杂志》
CSCD
北大核心
2002年第6期58-60,共3页
Chinese Journal of Zoonoses
