摘要
用MTT法测定了人肺癌细胞体外反应,可测率为85％,体外照射后存活率下降。单剂照射8Gy后,细胞存活下降占7/10例。照射2Gy后,对少数病例(2/10)的细胞有很显著的杀伤效应:1μg/ml顺铂作用后,存活下降占2/10,50μg/ml大多数病例存活下降至0.38—0.68。 HCT-8细胞体外照射MTT法测定与克隆存活分析法比较,一次照射2Gy以下,两种测定方法的结果较相近,增高剂量照射后两者结果相差较大。
Detection of radiosensitivity and chemosensitivity of fresh lung cancercells taken fromtpatients using MTT assay was carried out. The results showed that MTT assay is capable of detecting radiosensitivity and chemosensitivity of human solid tumor cells by careful selection and digestion.1 . Detectable rate of lung cancer cells by MTT assay was 85% (17 out of 20 cases). The failure was attributed to necrotic tissur in the specimens.2 . Survival rate of lung cancer cells treated with irradiation in vitro was determined using MTT assay. 7 out of 10 cases treated with a single dose of 8Gy irradiation showed reduction of ' A ' value. Severe killing effect observed in 2/10 casses treated with 2 Gy irradiation. The killing effect was depended on increase of irradiation dosage.3 . The response of human lung cancer cells to cis-platinum using MTT assay was dosedependent/ Remarkable killing effect was observed in 2 /10 cases treated with cis-platinum. Under the concentration of 1 μg/ml of, cis-platinum, suruival rate of lung cancer cells reduced to 0.3-0.52. In most cases, under the concentration of 50 μg/ml of cis-platinum, the survival rate of lung cancer cells was 0.38 - 0.68.4 . In campareing the survival rate of irradiated HCT-8 cells using MTT assay and clonogenic assay there was a similar result in a single dose of less of than 2 Gy irradiation . On the other hand, different effects of 4 and 6 Gy irradiation on cells was observed in there two assays, clonogenic survival fraction of cells treated by 4Gy and 6Gy irradiation declined to 0.8% and 0.0063% respectively.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1992年第6期437-439,共3页
Chinese Journal of Cancer
关键词
MTT法
放射敏感性
人肺癌细胞
MTT assay Rediosensitivity Chem osensitivity Human lung cancer cells HCT-8 cells
