摘要
用AgNOR定量法(计算AgNOR平均量/每核、数目、大小、位置及异形率)观察了78例肺癌(30例鳞癌、6例小细胞癌、31例腺癌、11例大细胞癌)病人的石蜡切片。结果表明,AgNOR颗粒有三种类型的形态特征:孤立圆形、细小弥漫型、粗大不规则型;每型肺癌有各自的一些形态特征;高分化腺癌细胞AgNOR平均数与其他类型肺癌相差非常显著(P<0.01);随着分化程度降低肺癌细胞AgNOR颗粒数增加、变细小或粗大不规则。作者认为,AgNORs颗粒数增加及不规则可能与基因调控、细胞增殖活性,核仁聚散等有关,对肺癌分型诊断有一定的帮助。
Using a silver staining technique, the nucleolar organizer region-associated protein (AgNOR) had been studied in paraffin sections of 78 lung cancers(30 squamous carcinomas, Q small cell carcinomas, 31 adenocarcinomas, and 11 large cell carcinomas). The quantitative indexes included the mean number of AgNOR per nucleus, size, number, and the percentage of irregularity of granules of AgNOR. The results showed that there were three features of the granule types: isolated and round, small and diffused, large and irregular. Each type of these pulmonary carcinoma cells showed different features, and there was a significant difference in the mean number of AgNORs per nucleus in lung cancer between well c'fferentiated adenocarcinomas and other types of lung cancers (P<0.01). The granules c AgNORs were sparse in well differentiated adenocarcinomas but numerous and irregular in poorly differentiated and undifferentiated ones. It was considered that the increasing number of AgNORs may be related to gen regulation, cell proliferative activity, aggregation and dispersion of NORs. It was suggested that the simple technique may be helpful in diagnosis and differentiationg pulmonary carcinomas.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1992年第4期270-272,285,共4页
Chinese Journal of Cancer
关键词
核仁组成区
组织化学
肺肿瘤
诊断
Nucleolar organizer region Pulmonary carcinoma Histochemistry DNA RNA
