摘要
用T细胞集落甲基纤维素微量培养方法,观实了25例胸部癌症病人围术期肿瘤去负荷前后外周血T细胞集落形成能力以及病人血清免疫抑制活性的动态变化。实验结果发现,25例病人肿瘤去负荷前T细胞集落产率为111.25±107.87/10~5,明显低于15例正常对照组(P<0.001)。肿瘤去负荷后,病人的T细胞集落产率提高至250.81±164.66/10~5(P<0.001),仍低于正常对照组。肿瘤去负荷前,病人血清对自身的T细胞集落产率有极为明显的抑制作用,T细胞产率为169.51±94.90/10~5,正常对照组为280.73±147.01/10~5,血清抑制率为45.15±18.06％。肿瘤去负荷后,T细胞集落产率提高至188.9±157.19/10~5,抑制率则下降为26.68±22.67％ P<0.001)。肿瘤去负荷后T细胞产率升高与血清抑制率下降两者呈负相关关系。
The dynamic change on colony forming unit-T lymphocyte (CFU-TL) in methylcellulose of lymphocytes and serum immunosuppressive activity before and after . discharging tumor load for 25 patients with thoracic cancer were surveyed. The results showed that the growth rate of CFU-TL before discharging tumor load was 111.25 ± 107.81/105(X±SD) and significantly tower than that ,of the normal control group (p< 0.001). The growth rate of CFU-TL was increased to 250.81 ±164.66/105 after discharging tumor load (p<0.001), but it was still lower than that of the normal control group. Sera from patients before discharging tumor load had a significant inhibitory effect on growth rate of CFrj-TL of autologus lymphocytes. The growth rate was 169.51±94.90/105 compared with 280.73±147.01/105 of the normal control group. The inhibitory rate was 45.15±18.06% . After discharging tumor load the growth rate was increased to 188.99± 157.19/105 and the inhibitory rate decreased to 26.68 ± 22.67% (p<0.001). The relation between the increase in the growth rate of CFU-TL and the reduction in inhibitory effect of sera after discharging tumor load showed significant negative correlation. The result showed that the capacity of CFU-TL in patients with thoracic cancer was significantly tower than that of normal because of there is inhibitory substance in serum released from tumor tissue. The radical operation could effectively decrease cell immunosuppression in patients with thoracic cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1992年第1期22-26,共5页
Chinese Journal of Cancer
关键词
围术期
T细胞集落
肿瘤
胸部
Thoracic cancer Perioperation T-cell colony
