摘要
人及动物组织癌变时,有胚胎性抗原的出现、同功酶谱的变化以及异位激素的分泌等现象,都说明肿瘤的发生与细胞反分化有关。许多癌基因的产物与细胞分化有关。某些癌基因编码的蛋白质属于蛋白激酶,对基因的表达起调控作用。 Hca-F25/CL-16A_3和Hca-F25/CL-A_2是两株分别具有高、低淋巴道转移能力的肿瘤克隆细胞系,它们都是从一个共同的肿瘤细胞株Hca-F25/L分离出来的。
Hca-F25/CL-16A3(16A3)and Hoa-F25/CL-A2(A2)are two mouse cancer cell clones, which are subcultures from a common tumor cell line Hca-F25/L, but have high (16A3) and low (A2) metastatic capacity respectively. A comparative study of chromatin chemical composition and phenol-soluble nonhistone proteins from 16A3, A2 and 615 mouse liver cells has been performed. The results of Chemical analysis of chromatin composition showed that the content of nonhistone chromatin protein of the cancer cell lines was higher than that of normal liver cells of 615 mice. The phosphorus determination of phenol-soluble nonhistone chromatin protein showed that the contents of phosphorus were higher in the two cancer cell lines than in normal liver cells of 615 mice. The pattern of phenol-soluble non histone proteirs on SDS-polyacrylamide gel electrophoresis revealed that there were differences in nonhistone protein of M.W. 60-40KD among 16A3o A2 and normal liver cells.
出处
《癌变·畸变·突变》
CAS
CSCD
1992年第1期22-25,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
