摘要
目的以透明质酸(hyaluronic acid,HA)对介孔二氧化硅纳米颗粒进行功能化(mesoporous silicana noparticle,MSN),并负载抗肿瘤药物顺铂(cisplatin,CDDP),制备CDDP@HA-MSN纳米药物,研究其形貌特征以及对人乳腺癌细胞(MDA-MB-231)的杀伤作用。方法通过扫描电镜(TEM)和动态光散射粒径分布仪(DLS)表征其形貌,通过MTT法检测其对MDA-MB-231细胞的杀伤作用。结果CDDP@HA-MSN平均粒径为150nm,载药量为18.7%,具有透明质酸酶响应性,细胞实验结果表明该纳米药物可明显增强对MDA-MB-231细胞的杀伤效果。结论成功制备了纳米药物CDDP@HA-MSN,能够显著增强抗肿瘤作用,有望提高对乳腺癌的靶向治疗效果。
Objective:To prepare the CDDP@HA-MSN nanomedicine by functionalizing Mesoporous Silica Nanoparticles(MSN)with hyaluronic acid(HA)and being loaded with cisplatin(CDDP),and to study the morphology and the effects on the survival rate of human breast cancer cells(MDA-MB-231).Methods:Its morphology was characterized by TEM and DLS,the proliferation inhibition of MDA-MB-231 cells was detected by MTT assay.Results:The average size of CDDP@HA-MSN nanoparticles was about 140 nm,and the drug load was 18.7%.The results of cell experiments showed that the nanomedicine significantly inhibited the proliferation of MDA-MB-231 cells.Conclusion:CDDP@HA-MSN can significantly enhance the killing ability of tumor cells,and it is expected to improve the efficacy of chemotherapy for breast cancer.
作者
刘伟坤
张萌
李炳龙
管西栋
孙明爽
何玉静
王春晓
赵宝昌
张吉梅
LIU Wei-kun;ZHANG Meng;LI Bing-long;GUAN Xi-dong;SUN Ming-shuang;HE Yu-jing;WANG Chun-xiao;ZHAO Bao-chang;ZHANG Ji-mei(Shandong First Medical University & Shandong Academy of Medical Sciences,Taian 271016,China)
出处
《泰山医学院学报》
CAS
2019年第11期801-805,共5页
Journal of Taishan Medical College
基金
国家级大学生创新创业训练计划项目(201810439076)
泰安市科技发展计划项目(2017NS0160,2018NS0101)
山东省医药卫生科技发展计划项目(2018WS126)
关键词
介孔二氧化硅
透明质酸
顺铂
纳米药物
乳腺癌
mesoporous silica nanoparticle
hyaluronan hyaluronic acid
cisplatin
nanomedicine
breast cancer
