摘要
从江西九连山霉烂毛红椿种子上分离种腐病原菌,采用形态学和分子生物学方法对其进行鉴定,并采用菌落直径法研究其在不同培养基、温度、pH以及多种碳氮源条件下的生物学特性。结果表明:从霉烂毛红椿种子上分离到一株对毛红椿种子具有强致病性(编号为JZF02)的真菌,经鉴定为厚垣镰刀菌(Fusarium chlamydosporum);该菌菌丝在不同培养基上的生长速率表现为PSA>PDA/MA>CA>CD;该菌能利用多种碳氮源,但铵盐不适合该菌生长;在0~40℃均能生长,最适生长温度为25~30℃;在pH5~12条件下均能生长,其中最适pH为8。本研究为掌握厚垣镰刀菌引起的毛红椿种子病害发生规律及采取防控措施提供了理论依据。
In this study,the pathogen isolated from the rotten seeds of Toona ciliata var. pubescens collected from Jiulianshan Mountains of Jiangxi Province and the morphological and molecular methods were used to identified the pathogen.And the biological characteristics of T. ciliata pathogen in different medium,temperature,pH and various carbon and nitrogen sources were studied by colony diameter method.The results showed that a fungus with strong pathogenicity (no.JZF 02) was isolated from mouldy T. ciliata seeds and identified as Fusarium chlamydosporum;The growth rate of fungal hyphae on different media showed PSA > PDA/MA > CA > CD;The strain could use a variety of carbon and nitrogen sources,but ammonium salt was not suitable for the growth of the bacteria;It could also grow at 0-40 ℃,and the optimum growth temperature was 25-30 ℃;Additionally,it could grow at pH 5-12,and the optimum pH was 8.This study provided a theoretical basis for mastering the occurrence rules of seed diseases caused by F. tabacum and taking prevention and control measures.
作者
任师杰
金泽枫
郭晓燕
尹思成
何光文
张露
REN Shijie;JIN Zefeng;GUO Xiaoyan;YIN Sicheng;HE Guangwen;ZHANG Lu(College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China;2011 Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《种子》
北大核心
2019年第10期47-51,共5页
Seed
基金
江西省自然科学基金项目(20171 BAB 204006)
江西省教育厅科学研究项目(GJJ 150434)
江西农业大学大学生创新创业训练计划项目(201810410093)
国家自然科学基金项目(31860202)
关键词
毛红椿
种腐病
厚垣镰刀菌
生物学特性
Toona ciliata var. Pubescens
seed rot pathogen
Fusarium chlamydosporum
biological characteristics
