摘要
目的:观察金佛手醇提液对哮喘小鼠肺组织中嗜酸性粒细胞(EOS)及肥大细胞(MC)的影响。方法:采用卵白蛋白(OVA)激发复制哮喘小鼠模型。将50只小鼠分为5组:正常对照组、模型组、金佛手醇提液高(30 g·kg^-1)、低(15 g·kg^-1)剂量组和阳性对照组(地塞米松,0.005 g·kg^-1),每组10只。灌胃给药,观察小鼠一般情况,肺组织内EOS及MC情况。结果:造模成功后,小鼠出现哮喘样表现。与正常对照组相比,模型组EOS、MC及EOS/白细胞比例均显著增加(P<0.01)。与模型组相比,金佛手醇提液高、低剂量组和阳性对照组EOS和MC及EOS/白细胞比例均显著下降(P<0.01)。金佛手醇提液高、低剂量组与阳性对照组比较EOS/白细胞比例、MC总计数差异无统计学意义(P>0.05)。结论:金佛手醇提液具有抗哮喘作用,其作用机制与抑制EOS炎症反应、抗MC脱颗粒密切相关。
Objective: To observe the effect of alcoholic extract of bergamot on eosinophils( EOS) and mast cells( MC) in lung tissue of asthmatic mice. Methods: The model of asthma in mice was induced by ovalbumin( OVA). Totally 50 mice were divided into 5 groups: the normal control group,the model group,alcohol extracts of bergamot high( 30 g·kg^-1) and low dose( 15 g·kg^-1)groups and the positive control group( dexamethasone,0. 005 g·kg^-1) with 10 ones in each group,and all the mice were with intragastric administration. The general conditions of mice,and EOS and MC in lung tissue were observed. Results: Compared with those in the normal control group,EOS,MC and EOS/WBC in the model group increased significantly( P < 0. 01). Compared with those in the model group,EOS,MC and EOS/WBC of alcohol extracts of bergamot high and low dose groups and the positive control group decreased significantly( P < 0. 01). There were no significant differences in EOS/WBC and MC between alcohol extracts of bergamot high and low dose groups and the positive control group( P > 0. 05). Conclusion: Alcohol extracts of bergamot has significant antiasthma effects,which may be related to the inhibition of EOS inflammatory reaction and MC degranulation.
作者
南李
周晓媚
方国英
徐孝平
陈小真
Nan Li;Zhou Xiaomei;Fang Guoying;Xu Xiaoping;Chen Xiaozhen(Hangzhou Women's Hospital, Hangzhou 310008, China;Laboratory Animal Research Center/Comparative Medical Research Institute, Zhejiang Chinese Medical University)
出处
《中国药师》
CAS
2019年第10期1787-1790,共4页
China Pharmacist
基金
杭州市卫生计生科技计划一般(A类)项目(编号:2015A44)
