摘要
以卟啉为原料合成了一种检测亚硝酸盐的近红外荧光探针,利用核磁共振氢谱和质谱对化合物结构进行了表征.研究了亚硝酸盐的浓度对探针荧光的影响,结果表明,随着亚硝酸盐浓度的增加,探针的荧光强度逐渐增强,且在0.0115~0.3000μmol/L浓度范围内,相对荧光强度与亚硝酸盐浓度之间具有良好的线性关系,线性方程为F/F0=5.9154c+1.0802,R2=0.9919,有很高的灵敏度,检测限为11.5nmol/L(S/N=3).选择性测试结果可知,探针与硝酸根离子反应后的相对荧光强度值为1.91,而与其他17种干扰物质反应后的相对荧光强度值均低于1.91,探针与亚硝酸盐反应后的相对荧光强度值为6.36,是探针与硝酸根离子反应后相对荧光强度值的3.3倍.抗干扰性测试结果可知,探针与亚硝酸盐反应后的相对荧光强度值为6.29,亚硝酸盐分别与其他18种干扰物质共同存在于溶液中时与探针反应后的相对荧光强度值在5.91~6.48变化,结果表明,探针对亚硝酸盐的检测具有良好的选择性和抗干扰能力.探针能成功地应用于不同规格的火腿肠和酱菜中亚硝酸盐的含量的测定,具有应用于实际样品中亚硝酸盐检测的潜力.
A near-infrared fluorescence probe was synthesized from porphyrins to detect nitrite,characterized by 1H NMR and MS spectra.The effect on probe of the concentrations of nitrite was studied,which showed that the fluorescence intensity of probe gradually increase by the increase of nitrite concentration,and the concentrations of nitrite range from 0.011 5 to 0.300 0 mol/L,it has a good linear relationship of the relative fluorescence intensity and nitrite concentration,the linear equation for the F/F 0=5.915 4+1.080 2 c,R 2=0.991 9,has a high sensitivity,the limit of detection is 11.5 nmol/L(S/N=3).Selective test result shows that the probe react with nitrate ion of the relative fluorescence intensity is 1.91,compared with other interfering substances,the relative fluorescence intensity of the reaction after less than 1.91,the relative fluorescence intensity value is 6.36 after the probe reacted with nitrite,is 3.3 times of the relative fluorescence intensity after the probe reacted with nitrate ion.Anti-interference experiment show that the probe and nitrite reaction after the relative fluorescence intensity value is 6.29,when nitrite and 18 other interfering substances coexist in solution,the relative fluorescence intensity varies between 5.91 and 6.48,the results show that the probe about the detection of nitrite with good selectivity and anti-jamming capacity.The probe can be successfully applied to the determination of nitrite content in different specifications of Ham sausage and Chinese pickles.
作者
杨贇山
吴振威
成奋民
张培盛
曾荣今
Yang Yunshan;Wu Zhenwei;Cheng Fenmin;Zhang Peisheng;Zeng Rongjin(Key Laboratory of Theoretical Organic Chemistry and Function Molecule,Ministry of Education,School of Chemistry and Chemical Engineering,Hunan University of Science and Technology,Xiangtan 411201,China)
出处
《湖南科技大学学报（自然科学版）》
CAS
北大核心
2019年第3期84-89,共6页
Journal of Hunan University of Science And Technology：Natural Science Edition
基金
国家自然科学基金资助项目(51873058)
关键词
卟啉
近红外
荧光探针
亚硝酸盐
porphyrins
near-infrared
fluorescence probe
nitrite
