摘要
以家蚕丝为原材料,采用两种溶解体系来调控蚕丝蛋白的分级组装,制备了不同尺寸的丝素蛋白纳米颗粒。对颗粒的形貌及结构等进行了测试分析,结果表明:采用LiBr-FA溶液体系制备的丝素蛋白纳米颗粒呈扁平状,当FA质量分数在5%时,丝素蛋白纳米颗粒呈无规卷曲构象;增加FA含量,促使β-折叠结构形成,丝素蛋白纳米颗粒结构更加规整;采用CaCl2/H2O/C2H5OH-FA溶解体系制备出的丝素蛋白纳米颗粒呈球状,颗粒的粒径均一、分布均匀,基本呈稳定的β-折叠结构。通过调控丝素蛋白自组装过程制备丝素蛋白纳米颗粒,整个过程不涉及有机溶剂的添加和复杂设备的使用,所制备的丝素蛋白纳米颗粒尺寸均匀、结构规整,可以进一步拓展丝素蛋白在药缓释领域的应用范围。
Using Bombyx mori silk as raw material, two dissolution systems were used to control the hierarchical assembly of silk fibroin, and different size of nanoparticles were prepared. The results showed that the nanoparticles prepared by LiBr-FA solution system were flattened. When the content of FA was 5%, the silk fibroin showed an irregular curly conformation. The structure of nanoparticles is more regular because of the formation of β-sheet structures with the increasing content of FA. The nanoparticles with β-sheet conformation prepared by CaCl 2/H 2O/C 2H 5OH-FA dissolution system are spherical, uniform, basically stable and evenly distributed. The method that preparing silk fibroin nanoparticles with uniform size and regular structure by regulating the assembly process without any addition of organic solvents and any use of complex instruments is relatively simple. Therefore, it is expected to further expand the application of silk protein in the field of drug sustained release.
作者
甘润生
张斐超
苏鑫贺
王英豪
张诗怡
GAN Runsheng;ZHANG Feichao;SU Xinhe;WANG Yinghao;ZHANG Shiyi(College of Textiles,Henan University of Engineering,Zhengzhou 450007,China)
出处
《河南工程学院学报（自然科学版）》
2019年第3期1-4,共4页
Journal of Henan University of Engineering：Natural Science Edition
基金
河南工程学院博士基金(D2015028)
关键词
自组装
丝素蛋白
纳米颗粒
self-assembly
silk fibroin
nanoparticles
