摘要
【目的】探讨血清外泌体miR-301a, miR-205, miR-376a, miR-222联合检测对神经胶质瘤的早期诊断价值。【方法】选择2016年1月至2019年1月本院收治的早期神经胶质瘤患者60例为观察组,选择同期于本院健康体检者60例为对照组。检测两组血清外泌体miR-301a, miR-205, miR-376a, miR-222相对表达量;用受试者工作特征曲线(ROC)的AUC分析血清外泌体miR-301a, miR-205, miR-376a, miR-222检测对早期神经胶质瘤的诊断价值。【结果】早期神经胶质瘤患者血清外泌体中miR-301a和miR-222的相对表达量均明显高于健康体检者(P<0.05);而早期神经胶质瘤患者血清外泌体中miR-205和miR-376a的相对表达量均明显低于健康体检者(P<0.05)。单向诊断早期神经胶质瘤患者与健康人群的血清外泌体miR-301a、miR-205、miR-376a、miR-222灵敏度分别为83%、72%、83%、87%,特异度分别为83%、73%、83%、87%。血清外泌体miR-301a、miR-205、miR-376a、miR-222联合检测对早期神经胶质瘤的诊断的灵敏度为85.00%,特异度为86.67%,准确度为85.83%。【结论】血清外泌体miR-301a、 miR-205、miR-376a、miR-222联合检测对早期神经胶质瘤有较高的诊断价值,可将其作为该病的潜在标志物。
【Objective】To investigate the early diagnostic value of combined detection of serum exosomes miR-301a,miR-205,miR-376a and miR-222 in glioma.【Methods】 Sixty patients with early glioma admitted to our hospital from January 2016 to January 2019 were included in the observation group and 60 healthy volunteers in the same period were selected as the control group.The relative expression levels of serum exosomes miR-301a,miR-205,miR-376a,and miR-222 were measured in the two groups.The diagnostic value of serum exosome miR-301a,miR-205,miR-376a and miR-222 for early gliomas was detected by AUC of receiver operating characteristic curve (ROC).【Results】The relative expression levels of serum exosomes miR-301a and miR-222 in patients with early glioma were significantly higher than those of healthy subjects (P<0.05).The relative expression levels of serum exosomes miR-205 and miR-376a in patients with early glioma were significantly lower than those of healthy subjects (P<0.05).The sensitivity of one-way diagnosis of serum exosomes miR-301a,miR-205,miR-376a or miR-222 in patients with early glioma and healthy people was 83%,72%,83% and 87%,respectively.The specificity were 83%,73%,83% and 87%,respectively.The sensitivity of the combination of serum exosomes miR-301a,miR-205,miR-376a and miR-222 for the diagnosis of early glioma was 85.00%,the specificity was 86.67%,and the accuracy was 85.83%.【Conclusion】The combined detection of the relative expression levels of serum exosomes miR-301a,miR-205,miR-376a and miR-222 provides high diagnostic value,which can be used as potential marker for the diagnosis of early glioma.
作者
郑忠涛
祝叶
李霞
ZHENG Zhong-tao;ZHU Ye;LI Xia(Haikou People's Hospital,Haikou 570208)
出处
《医学临床研究》
CAS
2019年第8期1483-1485,1488,共4页
Journal of Clinical Research
