摘要
目的:探讨高迁移率族蛋白B1(HMGB1)在糖尿病足患者外周血中的表达及临床意义。方法:收集92例2型糖尿病足患者静脉血标本作为试验组(糖尿病足组),另外取98例非糖尿病足2型糖尿病患者作为对照(糖尿病组),101例健康人作为正常对照组,采用酶联免疫吸附测定法检测受检对象外周静脉血中HMGB1、MMP9和AGE的表达量。结果:与正常对照组相比,糖尿病足组患者和糖尿病组患者血清中HMGB1、MMP9和AGE的表达量显著升高,差异有统计学意义(P<0.05);与糖尿病组患者相比,糖尿病足组患者HMGB1、MMP9和AGE表达量显著升高,差异有统计学意义(P<0.05);糖尿病足组患者血清中HMGB1的表达量与AGE(r^2=0.31)和MMP9(r^2=0.77)的表达量呈现正相关(P<0.05)。结论:糖尿病足患者外周血中HMGB1的表达量明显升高,其可能是糖尿病足发病机制中的重要参与者。
Objective The aim of this study was to determine HMGB1 expression profiles in type 2 diabetes patients without or with different forms of diabetic foot syndrome.Method Healthy volunteers(n=101) and type 2 diabetes patients without diabetic foot syndrome(n=98) and with diabetic foot syndrome were examined(n=92).Plasma concentrations of HMGB1, MMP9 and AGE expressions were measured by enzyme-linked immunosorbent assay.Results Diabetic patients both with and without foot syndrome had high HMGB1, AGE and MMP9 concentrations(all three group, P<0.05) when compared with the nondiabetic volunteers.However, subjects with diabetic foot syndrome had upregulated HMGB1, MMP9 and AGE expressions when compared with the type 2 diabetes group.In patients with diabetic foot syndrome, a significant association was observed between the expression of HMGB1 and AGE(r^2=0.31) as well as serum MMP9(r^2=0.77) concentration(P<0.05).Conclusion Patients with type 2 diabetes and different forms of diabetic foot syndrome have an altered expression of HMGB1, and HMGB1 could contribute to the pathogenesis of different forms of diabetic foot syndrome.
出处
《吉林医学》
CAS
2019年第9期1943-1946,共4页
Jilin Medical Journal
基金
重庆市卫生和计划生育委员会2017年中医药科技项目[项目编号:ZY201702080]
重庆市卫生和计划生育委员会中医药科技项目[项目编号:zy201602048]
